RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)
  • Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR19523] to BACE1
  • Suitable for: IHC-P
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523]
    See all BACE1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR19523] to BACE1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment within Mouse BACE1 aa 50-350. The exact sequence is proprietary.
    Database link: P56818

  • Positive control

    • IHC-P: normal mouse brain tissue sections

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab225307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.

  • Tissue specificity

    Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.

  • Sequence similarities

    Belongs to the peptidase A1 family.

  • Domain

    The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt

  • Database links

    • Alternative names

      • APP beta secretase antibody
      • Asp 2 antibody
      • ASP2 antibody
      • Aspartyl protease 2 antibody
      • BACE 1 antibody
      • BACE antibody
      • BACE1 antibody
      • BACE1_HUMAN antibody
      • Beta secretase 1 antibody
      • Beta secretase antibody
      • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
      • Beta site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta site amyloid precursor protein cleaving enzyme antibody
      • Beta site APP cleaving enzyme 1 antibody
      • Beta site APP cleaving enzyme antibody
      • Beta-secretase 1 antibody
      • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
      • Beta-site APP cleaving enzyme 1 antibody
      • FLJ90568 antibody
      • HSPC104 antibody
      • Memapsin 2 antibody
      • Memapsin-2 antibody
      • Memapsin2 antibody
      • Membrane associated aspartic protease 2 antibody
      • Membrane-associated aspartic protease 2 antibody
      • Transmembrane aspartic proteinase Asp2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)

      IHC image of BACE1 staining in a section of formalin-fixed paraffin-embedded normal mouse brain.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110°C for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225307 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)
      Alexa Fluor® 647 Anti-BACE1 antibody [EPR19523] (ab225307)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab225307? Please let us know so that we can cite the reference in this datasheet.

    ab225307 has not yet been referenced specifically in any publications.

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