Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (ab187602)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (ab187602)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [B2M01] to beta 2 Microglobulin
    • Suitable for: ICC/IF
    • Reacts with: Human, Recombinant fragment
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2a

    Overview

    • Product name

      Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01]
      See all beta 2 Microglobulin primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [B2M01] to beta 2 Microglobulin

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Specificity

      ab187602 reacts with beta 2 microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin.

    • Tested Applications & Species

      Application Species
      IHC-P
      Recombinant fragment
      See all applications and species data

    • Immunogen

      Full length native protein (purified) corresponding to Human beta 2 Microglobulin.
      Database link: P61769

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab187602 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Recombinant fragment
    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Function

      Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

    • Involvement in disease

      Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
      Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

    • Sequence similarities

      Belongs to the beta-2-microglobulin family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      Glycation of Ile-21 is observed in long-term hemodialysis patients.

    • Cellular localization

      Secreted. Detected in serum and urine.
    • Information by UniProt

    • Database links

      • Alternative names

        • B2M antibody
        • B2MG_HUMAN antibody
        • Beta 2 microglobin antibody
        • Beta 2 microglobulin antibody
        • Beta 2 microglobulin precursor antibody
        • Beta chain of mhc class 1 proteins antibody
        • Beta chain of MHC class I molecules antibody
        • Beta-2-microglobulin form pI 5.3 antibody
        • CDABP0092 antibody
        • Hdcma22p antibody
        • IMD43 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (ab187602)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (ab187602)

        Immunocytochemistry/ Immunofluorescence of Human beta 2 Microglobulin-transfected canine cells labeled A)  with ab187602 and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2µg/ml.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab187602? Please let us know so that we can cite the reference in this datasheet.

      ab187602 has not yet been referenced specifically in any publications.

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