Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)

    Key features and details

    • Alexa Fluor® 647 Rat monoclonal [M1/70] to CD11b
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    Carrier Free Unconjugated

    Overview

    • Product name

      Alexa Fluor® 647 Anti-CD11b antibody [M1/70]
      See all CD11b primary antibodies

    • Description

      Alexa Fluor® 647 Rat monoclonal [M1/70] to CD11b

    • Host species

      Rat

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rabbit, Human

    • Positive control

      • ICC/IF: RAW246.7 cells

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab197702 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/100.
    Notes
    ICC/IF
    1/100.

    Target

    • Function

      Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

    • Tissue specificity

      Predominantly expressed in monocytes and granulocytes.

    • Involvement in disease

      Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.

    • Domain

      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • antigen CD11b (p170) antibody
        • Antigen CD11b p170 antibody
        • CD11 antigen like family member B antibody
        • CD11 antigen-like family member B antibody
        • CD11b antibody
        • CD11b/CD18 antibody
        • CD49d antibody
        • Cell surface glycoprotein MAC-1 subunit alpha antibody
        • Complement component 3 receptor 3 subunit antibody
        • Complement Component Receptor 3 Alpha antibody
        • Complement receptor type 3 antibody
        • Complement receptor type 3, alpha subunit antibody
        • CR 3 alpha chain (CR3A) antibody
        • CR 3 alpha chain antibody
        • CR-3 alpha chain antibody
        • CR3 antibody
        • CR3A antibody
        • F730045J24Rik antibody
        • Integrin Alpha M antibody
        • Integrin alpha M chain antibody
        • Integrin alpha-M antibody
        • Integrin beta 2 alpha subunit antibody
        • Integrin subunit alpha M antibody
        • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
        • ITAM_HUMAN antibody
        • ITGAM antibody
        • Leukocyte adhesion receptor MO1 antibody
        • Ly-40 antibody
        • MAC 1 antibody
        • Mac-1a antibody
        • MAC1 antibody
        • Mac1, alpha subunit antibody
        • MAC1A antibody
        • Macrophage antigen alpha polypeptide antibody
        • MGC117044 antibody
        • Mo1 antibody
        • Mo1, alpha subunit antibody
        • MO1A antibody
        • Neutrophil adherence receptor alpha M subunit antibody
        • Neutrophil adherence receptor antibody
        • SLEB6 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)

        ab197702 staining CD11b in Raw264.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197702 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      References (1)

      Publishing research using ab197702? Please let us know so that we can cite the reference in this datasheet.

      ab197702 has been referenced in 1 publication.

      • Hanot Mambres D  et al. Identification of Immune Effectors Essential to the Control of Primary and Secondary Intranasal Infection with Brucella melitensis in Mice. J Immunol 196:3780-93 (2016). PubMed: 27036913

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