Alexa Fluor® 647 Anti-CD177 antibody [MEM-166] (ab187590)
![Flow Cytometry - Alexa Fluor® 647 Anti-CD177 antibody [MEM-166] (ab187590)](https://www.abcam.com/ps/products/187/ab187590/Images/ab187590-1-anti-cd177-antibody-mem166-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-CD177 antibody [MEM-166] (ab187590)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [MEM-166] to CD177
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
-
Product name
Alexa Fluor® 647 Anti-CD177 antibody [MEM-166]
See all CD177 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [MEM-166] to CD177 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD177. Human granulocytes.
-
Positive control
- Flow Cyt: Human blood cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-166 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab187590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Target
-
Tissue specificity
Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 57126 Human
- Omim: 162860 Human
- SwissProt: Q8N6Q3 Human
- Unigene: 232165 Human
-
Alternative names
- CD177 antibody
- CD177 antigen antibody
- CD177 molecule antibody
see all
Images
-
Flow Cytometry - Alexa Fluor® 647 Anti-CD177 antibody [MEM-166] (ab187590)Flow cytometry analysis of human peripheral blood labeling CD177 using ab187590 at 4 µL/100 µL whole blood. Surface staining.
-
![Flow Cytometry - Alexa Fluor® 647 Anti-CD177 antibody [MEM-166] (ab187590)](https://www.abcam.com/ps/products/187/ab187590/Images/ab187590-2-anti-cd177-antibody-mem166-flow-cytometry-peripheral-blood-human-histogram.jpg)
Flow Cytometry - Alexa Fluor® 647 Anti-CD177 antibody [MEM-166] (ab187590)Flow cytometry analysis of human peripheral blood labeling CD177 using ab187590 at 4 µL/100 µL whole blood. CD177-positive neutrophil granulocytes (Red) are separated from CD177-negative lymphocytes (Black, dashed line). Surface staining.
Datasheets and documents
References (0)
ab187590 has not yet been referenced specifically in any publications.
