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    alexa-fluor-647-cd3-antibody-okt3-ab253269.pdf

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Immunology Adaptive Immunity T Cells CD
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Alexa Fluor® 647 Anti-CD3 antibody [OKT3] (ab253269)

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Flow Cytometry - Alexa Fluor® 647 Anti-CD3 antibody [OKT3] (ab253269)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [OKT3] to CD3
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 700 APC Biotin Carrier Free FITC redFluor™ 710 Unconjugated

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    Overview

    • Product name

      Alexa Fluor® 647 Anti-CD3 antibody [OKT3]
      See all CD3 primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [OKT3] to CD3
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • Epitope

      extracellular
    • Positive control

      • Flow Cyt: Human peripheral blood leukocytes.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Purification notes

      The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
    • Clonality

      Monoclonal
    • Clone number

      OKT3
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    Associated products

    • Alternative Versions

      • redFluor™ 710 Anti-CD3 antibody [OKT3] (ab243296)
      • Alexa Fluor® 488 Anti-CD3 antibody [OKT3] (ab253249)
      • Alexa Fluor® 700 Anti-CD3 antibody [OKT3] (ab253263)
      • Biotin Anti-CD3 antibody [OKT3] (ab272405)
      • Anti-CD3 antibody [OKT3] (ab86883)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab253269 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 4µl for 106 cells.

    Or 100 µl of whole blood.

    Notes
    Flow Cyt
    Use 4µl for 106 cells.

    Or 100 µl of whole blood.

    Target

    • Function

      The CD3 complex mediates signal transduction.
    • Involvement in disease

      Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    • Sequence similarities

      Contains 1 ITAM domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P04234 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 915 Human
      • Entrez Gene: 916 Human
      • Entrez Gene: 917 Human
      • Entrez Gene: 919 Human
      • Omim: 186740 Human
      • Omim: 186780 Human
      • Omim: 186790 Human
      • Omim: 186830 Human
      • SwissProt: P04234 Human
      • SwissProt: P07766 Human
      • SwissProt: P09693 Human
      • SwissProt: P20963 Human
      • Unigene: 504048 Human
      see all
    • Alternative names

      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • Cd247 antibody
      • CD247 antigen antibody
      • CD247 antigen, zeta subunit antibody
      • CD247 molecule antibody
      • CD3 antibody
      • CD3 antigen, delta subunit antibody
      • CD3 delta antibody
      • CD3 epsilon antibody
      • CD3 eta antibody
      • CD3 gamma antibody
      • CD3 molecule delta polypeptide antibody
      • CD3 molecule, epsilon polypeptide antibody
      • CD3 molecule, gamma polypeptide antibody
      • CD3 zeta antibody
      • CD3-DELTA antibody
      • CD3d antibody
      • CD3D antigen delta polypeptide antibody
      • CD3d antigen, delta polypeptide (TiT3 complex) antibody
      • CD3d molecule delta antibody
      • CD3d molecule delta CD3 TCR complex antibody
      • CD3d molecule, delta (CD3-TCR complex) antibody
      • CD3D_HUMAN antibody
      • CD3E antibody
      • CD3e antigen antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule epsilon CD3 TCR complex antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3epsilon antibody
      • CD3G antibody
      • CD3g antigen antibody
      • CD3G antigen gamma polypeptide antibody
      • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
      • CD3g molecule gamma antibody
      • CD3g molecule gamma CD3 TCR complex antibody
      • CD3g molecule, gamma (CD3-TCR complex) antibody
      • CD3H antibody
      • CD3Q antibody
      • CD3Z antibody
      • CD3zeta antibody
      • Ctg3 antibody
      • FLJ17620 antibody
      • FLJ17664 antibody
      • FLJ18683 antibody
      • FLJ79544 antibody
      • FLJ94613 antibody
      • IMD19 antibody
      • Leu-4 antibody
      • MGC138597 antibody
      • OKT3, delta chain antibody
      • OTTHUMP00000032544 antibody
      • T cell receptor antibody
      • T cell receptor T3 delta chain antibody
      • T cell receptor T3 gamma chain antibody
      • T cell receptor T3 zeta chain antibody
      • T cell receptor zeta chain antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 antibody
      • T cell surface glycoprotein CD3 delta chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T cell surface glycoprotein CD3 gamma chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, delta subunit of T3 antibody
      • T-cell antigen receptor complex, epsilon subunit of T3 antibody
      • T-cell antigen receptor complex, gamma subunit of T3 antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 delta chain antibody
      • T-cell receptor T3 gamma chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 delta chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface glycoprotein CD3 gamma chain antibody
      • T3 antibody
      • T3d antibody
      • T3e antibody
      • T3g antibody
      • T3z antibody
      • TCRE antibody
      • TCRk antibody
      • Tcrz antibody
      • TCRzeta antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 647 Anti-CD3 antibody [OKT3] (ab253269)
      Flow Cytometry - Alexa Fluor® 647 Anti-CD3 antibody [OKT3] (ab253269)

      Flow Cytometry analysis of human peripheral blood leukocytes labeling CD3 with ab253269. Surface staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab253269? Please let us know so that we can cite the reference in this datasheet.

    ab253269 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab253269.
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