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    alexa-fluor-647-cd41-antibody-m148-ab233054.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Integrins
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Alexa Fluor® 647 Anti-CD41 antibody [M148] (ab233054)

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Flow Cytometry - Alexa Fluor® 647 Anti-CD41 antibody [M148] (ab233054)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [M148] to CD41
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Product image
    Alexa Fluor® 488 Anti-CD41 antibody [M148] (ab233053)

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    Overview

    • Product name

      Alexa Fluor® 647 Anti-CD41 antibody [M148]
      See all CD41 primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [M148] to CD41
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Specificity

      This antibody binds to some medulloblastoma and neuroblastoma cells and rhabdomyosarcoma and certain other solid tumours. No binding observed to marrow constituents other than platelets and megakaryocytes.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human CD41. Homogenized human medulloblastoma tissue.
      Database link: P08514

    • Positive control

      • Flow cyt: HEL 92.1.7 cells
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 30% Glycerol, 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      M148
    • Myeloma

      P3x63-Ag8.653
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Integrins
      • Cardiovascular
      • Blood
      • Platelets
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Alpha
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Murine Lineage Negative
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Thrombocytic Lineage

    Associated products

    • Alternative Versions

      • Anti-CD41 antibody [M148] (ab11024)
      • FITC Anti-CD41 antibody [M148] (ab19708)
      • Alexa Fluor® 488 Anti-CD41 antibody [M148] (ab233053)
      • Anti-CD41 antibody [M148] - BSA and Azide free (ab233170)

    Applications

    Our Abpromise guarantee covers the use of ab233054 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/12500.

    Target

    • Function

      Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
    • Tissue specificity

      Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
    • Involvement in disease

      Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P08514 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3674 Human
      • Omim: 607759 Human
      • SwissProt: P08514 Human
      • Unigene: 411312 Human
      • Alternative names

        • antigen CD41 antibody
        • BDPLT16 antibody
        • BDPLT2 antibody
        • CD41 antibody
        • CD41B antibody
        • form 2 antibody
        • GP2B antibody
        • GPalpha IIb antibody
        • GPIIb antibody
        • GT antibody
        • GTA antibody
        • HPA3 antibody
        • Integrin alpha 2b antibody
        • Integrin alpha IIb antibody
        • Integrin alpha-IIb light chain antibody
        • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
        • ITA2B_HUMAN antibody
        • Itga2b antibody
        • ITGAB antibody
        • platelet fibrinogen receptor, alpha subunit antibody
        • platelet glycoprotein IIb of IIb/IIIa complex antibody
        • Platelet membrane glycoprotein IIb antibody
        • platelet specific antigen BAK antibody
        • PPP1R93 antibody
        see all

      Images

      • Flow Cytometry - Alexa Fluor® 647 Anti-CD41 antibody [M148] (ab233054)
        Flow Cytometry - Alexa Fluor® 647 Anti-CD41 antibody [M148] (ab233054)

        Overlay histogram showing left HEL92.1.7 positive cells and right negative Jurkat cells stained with ab233054 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab233054)  (1x106 in 100 μl at 0.04 μg/ml (1/12500)) for 30 min on ice. Isotype control antibody (black line) was mouse IgG2a kappa; Alexa Fluor® 647  used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab233054? Please let us know so that we can cite the reference in this datasheet.

      ab233054 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab233054.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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