Alexa Fluor® 647 Anti-CD55 antibody [MEM-118] (ab239274)
![Flow Cytometry - Alexa Fluor® 647 Anti-CD55 antibody [MEM-118] (ab239274)](https://www.abcam.com/ps/products/239/ab239274/Images/ab239274-319937-ab239274.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-CD55 antibody [MEM-118] (ab239274)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [MEM-118] to CD55
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgM
Overview
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Product name
Alexa Fluor® 647 Anti-CD55 antibody [MEM-118]
See all CD55 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [MEM-118] to CD55 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates
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Immunogen
Tissue, cells or virus corresponding to Human CD55. (HPB-ALL human T cell line).
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Epitope
ab239274 recognizes an epitope in SCR4 domain of CD55. -
Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 8.0
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
MEM-118 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab239274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. (or 100 μl of whole blood). |
Target
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Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. -
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains. -
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. -
Post-translational
modificationsThe Ser/Thr-rich domain is heavily O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
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Alternative names
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 647 Anti-CD55 antibody [MEM-118] (ab239274)
Flow cytometric analysis of human peripheral blood cells labeling CD55 with ab239274. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239274 has not yet been referenced specifically in any publications.
