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    alexa-fluor-647-cd68-antibody-fa-11-ab201845.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)

    Key features and details

    • Alexa Fluor® 647 Rat monoclonal [FA-11] to CD68
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2a

    You may also be interested in

    Primary
    Product image
    Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
    Protein
    Recombinant human CD68 protein (Fc Chimera Active) (ab214829)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 647 Anti-CD68 antibody [FA-11]
      See all CD68 primary antibodies
    • Description

      Alexa Fluor® 647 Rat monoclonal [FA-11] to CD68
    • Host species

      Rat
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD68.

    • Positive control

      • ICC/IF: RAW246.7 cells
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol, 1% BSA
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      FA-11
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells

    Associated products

    • Alternative Versions

      • Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
      • Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
      • HRP Anti-CD68 antibody [FA-11] (ab216526)
      • PE Anti-CD68 antibody [FA-11] (ab216701)
      • PerCP Anti-CD68 antibody [FA-11] (ab220509)
      • APC Anti-CD68 antibody [FA-11] (ab221251)
      • Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
      • Anti-CD68 antibody [FA-11] (ab53444)
    • Recombinant Protein

      • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)

    Applications

    Our Abpromise guarantee covers the use of ab201845 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/200.

    This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min)

    Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities

      Belongs to the LAMP family.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Target information above from: UniProt accession P34810 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12514 Mouse
      • SwissProt: P31996 Mouse
      • Unigene: 15819 Mouse
      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)

        ab201845 staining CD68 in RAW264.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201845 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

         

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab201845? Please let us know so that we can cite the reference in this datasheet.

      ab201845 has been referenced in 4 publications.

      • Swiderski K  et al. Deletion of suppressor of cytokine signaling 3 (SOCS3) in muscle stem cells does not alter muscle regeneration in mice after injury. PLoS One 14:e0212880 (2019). PubMed: 30811469
      • Ordonez AA  et al. Radiosynthesis and PET Bioimaging of 76Br-Bedaquiline in a Murine Model of Tuberculosis. ACS Infect Dis N/A:N/A (2019). PubMed: 31345032
      • Wen G  et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
      • Xu S  et al. Ecto-5'-nucleotidase (CD73) attenuates inflammation after spinal cord injury by promoting macrophages/microglia M2 polarization in mice. J Neuroinflammation 15:155 (2018). PubMed: 29788960

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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