Recombinant Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)](https://www.abcam.com/ps/products/237/ab237365/Images/ab237365-2-IHCAP00417431.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR21769] to CD8 alpha
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]
See all CD8 alpha primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR21769] to CD8 alpha -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-P Human
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Immunogen
Recombinant fragment within Mouse CD8 alpha aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01731 -
Positive control
- Flow Cyt: C57BL/6 mouse splenocytes. IHC-P: Normal mouse spleen tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21769 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD8 alpha antibody [EPR21769] (ab217344)
- Anti-CD8 alpha antibody [EPR21769] - BSA and Azide free (ab230156)
- Alexa Fluor® 488 Anti-CD8 alpha antibody [EPR21769] (ab237364)
- FITC Anti-CD8 alpha antibody [EPR21769] (ab237367)
- APC Anti-CD8 alpha antibody [EPR21769] (ab237368)
- Alexa Fluor® 594 Anti-CD8 alpha antibody [EPR21769] (ab277939)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/1000.
|
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt
1/1000. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 925 Human
- Entrez Gene: 12525 Mouse
- Omim: 186910 Human
- SwissProt: P01732 Human
- SwissProt: P01731 Mouse
- Unigene: 85258 Human
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)
IHC image of CD8 alpha staining in a section of formalin-fixed paraffin-embedded normal mouse spleen.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins, performed on a Leica BOND™. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab237365 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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![Flow Cytometry - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)](https://www.abcam.com/ps/products/237/ab237365/Images/ab237365-1-ab237365FLOW1.jpg)
Flow Cytometry - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)C57BL/6 mouse splenocytes stained with ab237365 (right) or Rabbit IgG Alexa Fluor® 647 (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab237365) or Rabbit IgG 647 Isotype (1x106 in 100 µl at 1:1000 (0.5 µg/ml)) for 30 mins on ice. The cells were simultaneously stained with CD4 PerCP conjugated antibody.
Acquisition of >50,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on viable lymphocytes.
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![Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)](https://www.abcam.com/ps/products/237/ab237365/Images/ab237365-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (ab237365)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab237365 has not yet been referenced specifically in any publications.