Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR23105-121] to CD9
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Product nameAlexa Fluor® 647 Anti-CD9 antibody [EPR23105-121]
See all CD9 primary antibodies
DescriptionAlexa Fluor® 647 Rabbit monoclonal [EPR23105-121] to CD9
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: Flow Cyt, ICCmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CD9 aa 100-200. The exact sequence is proprietary.
Database link: P21926
- ICC: HCT116 cells. Flow Cyt: Human PBMCs.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68.98% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab267503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in HCT116 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
FunctionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificityExpressed by a variety of hematopoietic and epithelial cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
- Information by UniProt
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab267503 (right panel) or Rabbit IgG (monoclonal) Alexa Fluor® 647 isotype (left panel).
PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab267503) or Rabbit IgG (monoclonal) Alexa Fluor® 647 isotype (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 mins on ice.
Acquisition of >30000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
Events were gated on live single cells.
Immunofluorescence staining of CD9 in HCT116 cells using ab267503. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab267503 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HCT116 cells fixed with 4% formaldehyde (10 min).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab267503 has not yet been referenced specifically in any publications.