Key features and details
- Alexa Fluor® 647 Rabbit monoclonal [EPR15620] to CDC42
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Product nameAlexa Fluor® 647 Anti-CDC42 antibody [EPR15620]
See all CDC42 primary antibodies
DescriptionAlexa Fluor® 647 Rabbit monoclonal [EPR15620] to CDC42
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human CDC42 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P60953
- ICC/IF: Jurkat cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-CDC42 antibody [EPR15620] (ab187643)
- Alexa Fluor® 488 Anti-CDC42 antibody [EPR15620] (ab214952)
- HRP Anti-CDC42 antibody [EPR15620] (ab215810)
- Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)
- APC Anti-CDC42 antibody [EPR15620] (ab225345)
- PE Anti-CDC42 antibody [EPR15620] (ab225346)
Our Abpromise guarantee covers the use of ab215234 in the following tested applications.
This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min)
FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Cellular localizationCell membrane.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
ab215234 staining CDC42 in Jurkat cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215234 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 4% formaldehyde (10 min).
ab215234 has not yet been referenced specifically in any publications.