Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [37BA11] to Cytochrome C
    • Suitable for: ICC/IF
    • Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2a

    Overview

    • Product name

      Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11]
      See all Cytochrome C primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [37BA11] to Cytochrome C

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Mouse, Rat, Cow, Human, Caenorhabditis elegans

    • Immunogen

      Full length native protein (purified) corresponding to Cow Cytochrome C. Bovine heart Cytochrome C.

    • Positive control

      • ICC/IF: HeLa cells.

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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    Properties

    Applications

    Our Abpromise guarantee covers the use of ab198583 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

    Target

    • Function

      Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
      Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

    • Involvement in disease

      Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

    • Sequence similarities

      Belongs to the cytochrome c family.

    • Post-translational
      modifications

      Binds 1 heme group per subunit.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CYC antibody
      • CYC_HUMAN antibody
      • CYCS antibody
      • Cytochrome c antibody
      • Cytochrome c somatic antibody
      • HCS antibody
      • THC4 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11] (ab198583)

      ab198583 staining Cytochrome C in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198583 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    References (2)

    Publishing research using ab198583? Please let us know so that we can cite the reference in this datasheet.

    ab198583 has been referenced in 2 publications.

    • Zhou H  et al. The oncolytic compound LTX-401 targets the Golgi apparatus. Cell Death Differ 23:2031-2041 (2016). PubMed: 27588704
    • Zhou H  et al. The oncolytic peptide LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization. Oncotarget 6:26599-614 (2015). PubMed: 26378049

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