Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)](https://www.abcam.com/ps/products/233/ab233045/Images/ab233045-1-ICCAP46556671.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG2b
Overview
-
Product name
Alexa Fluor® 647 Anti-DDIT3 antibody [9C8]
See all DDIT3 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein within DDIT3. The exact sequence is proprietary. A bacterially expressed, mouse DDIT3 fusion protein.
-
Epitope
ab11419 has been shown to recognize an epitope in the N-terminal region of DDIT3. -
Positive control
- ICC/IF: Tunicamycin (1.5uM, 6hr) treated HeLa cells.
-
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9C8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab233045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. This product gave a positive signal in Tunicamycin (1.5uM, 6hr) treated HeLa fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Target
-
Function
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. -
Involvement in disease
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1649 Human
- Omim: 126337 Human
- SwissProt: P35638 Human
- Unigene: 505777 Human
- Unigene: 728989 Human
-
Alternative names
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
ab233045 staining DDIT3 in HeLa cells +/- Tunicamycin (1.5uM, 6hr). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab233045 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa treated with Tunicamycin (1.5uM, 6hr) cells fixed with 100% methanol (5 min).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab233045 has not yet been referenced specifically in any publications.
