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Epigenetics and Nuclear Signaling Transcription Other factors
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Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2b

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    Product image
    Human DDIT3 knockout HeLa cell line (ab265760)
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    Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

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    Overview

    • Product name

      Alexa Fluor® 647 Anti-DDIT3 antibody [9C8]
      See all DDIT3 primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Fusion protein within DDIT3. The exact sequence is proprietary. A bacterially expressed, mouse DDIT3 fusion protein.

    • Epitope

      ab11419 has been shown to recognize an epitope in the N-terminal region of DDIT3.
    • Positive control

      • ICC/IF: Tunicamycin (1.5uM, 6hr) treated HeLa cells.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 30% Glycerol, 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      9C8
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Stem Cells
      • Embryonic Stem Cells
      • Intracellular
      • Developmental Biology
      • Embryogenesis
      • Embryonic stem cells
      • Surface molecules
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Types of disease
      • Metabolic disorders

    Associated products

    • Alternative Versions

      • Anti-DDIT3 antibody [9C8] (ab11419)
      • Anti-DDIT3 antibody [9C8] - BSA and Azide free (ab233121)

    Applications

    Our Abpromise guarantee covers the use of ab233045 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    This product gave a positive signal in Tunicamycin (1.5uM, 6hr) treated HeLa fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Target

    • Function

      Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA.
    • Involvement in disease

      Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS.
    • Sequence similarities

      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P35638 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1649 Human
      • Omim: 126337 Human
      • SwissProt: P35638 Human
      • Unigene: 505777 Human
      • Unigene: 728989 Human
      • Alternative names

        • C/EBP homologous protein antibody
        • C/EBP Homology Protein antibody
        • C/EBP zeta antibody
        • C/EBP-homologous protein 10 antibody
        • C/EBP-homologous protein antibody
        • CCAAT/enhancer binding protein homologous protein antibody
        • CEBPZ antibody
        • CHOP 10 antibody
        • CHOP antibody
        • CHOP-10 antibody
        • CHOP10 antibody
        • DDIT 3 antibody
        • DDIT-3 antibody
        • Ddit3 antibody
        • DDIT3_HUMAN antibody
        • DNA Damage Inducible Transcript 3 antibody
        • DNA damage-inducible transcript 3 protein antibody
        • GADD 153 antibody
        • GADD153 antibody
        • Growth Arrest and DNA Damage Inducible Protein 153 antibody
        • Growth arrest and DNA damage inducible protein GADD153 antibody
        • Growth arrest and DNA damage-inducible protein GADD153 antibody
        • MGC4154 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)

        ab233045 staining DDIT3 in HeLa cells +/- Tunicamycin (1.5uM, 6hr). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab233045 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        This product also gave a positive signal under the same testing conditions in HeLa treated with Tunicamycin (1.5uM, 6hr) cells fixed with 100% methanol (5 min).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab233045? Please let us know so that we can cite the reference in this datasheet.

      ab233045 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab233045.
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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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