Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)](https://www.abcam.com/ps/products/233/ab233045/Images/ab233045-1-ICCAP46556671.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG2b
Overview
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Product name
Alexa Fluor® 647 Anti-DDIT3 antibody [9C8]
See all DDIT3 primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [9C8] to DDIT3 -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Fusion protein within DDIT3. The exact sequence is proprietary. A bacterially expressed, mouse DDIT3 fusion protein.
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Epitope
ab11419 has been shown to recognize an epitope in the N-terminal region of DDIT3. -
Positive control
- ICC/IF: Tunicamycin (1.5uM, 6hr) treated HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9C8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in Tunicamycin (1.5uM, 6hr) treated HeLa fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
| Notes |
|---|
|
ICC/IF
1/100. This product gave a positive signal in Tunicamycin (1.5uM, 6hr) treated HeLa fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Target
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Function
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. -
Involvement in disease
Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1649 Human
- Omim: 126337 Human
- SwissProt: P35638 Human
- Unigene: 505777 Human
- Unigene: 728989 Human
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Alternative names
- C/EBP homologous protein antibody
- C/EBP Homology Protein antibody
- C/EBP zeta antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDIT3 antibody [9C8] (ab233045)
ab233045 staining DDIT3 in HeLa cells +/- Tunicamycin (1.5uM, 6hr). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab233045 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa treated with Tunicamycin (1.5uM, 6hr) cells fixed with 100% methanol (5 min).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233045 has not yet been referenced specifically in any publications.