Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR3864] to ERG
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Product nameAlexa Fluor® 647 Anti-ERG antibody [EPR3864]
See all ERG primary antibodies
DescriptionAlexa Fluor® 647 Rabbit monoclonal [EPR3864] to ERG
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- ICC/IF: THP-1 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 8.90 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)
This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)
FunctionTranscriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Involvement in diseaseDefects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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For mouse retina immunostaining, eyes were collected at the indicated time points and fixed in 4% PFA in PBS for 1 h at room temperature (RT). After two PBS washes, retinas were micro-dissected and stained. Briefly, retinas were blocked and permeabilized with 0.3% Triton X-100, 3% fetal bovine serum (FBS) and 3% donkey serum in PBS. Samples were then washed twice in PBLEC buffer (1 mM CaCl2, 1 mM MgCl2, 1 mM MnCl2 and 1% Triton X-100 in PBS). Biotinylated isolectinB4 or primary antibodies (Panel a, ab196149 1/100 dilution) were diluted in PBLEC buffer and tissues were incubated in this solution for 2 h at RT or overnight at 4 °C. After five washes in blocking solution diluted 1:2, samples were incubated for 1 h at RT with Alexa-conjugated secondary antibody. After two washes in PBS, retinas were mounted with Fluoromount-G. To detect EdU-labeled DNA, an additional step was performed before mounting using the Click-It EdU kit.
Dll4/Notch signalling inhibition induces context-dependent proliferative effects. a–d Confocal micrographs of the postnatal retinal vasculature from animals treated at P5 with IgG (control) or anti-Dll4 for 24 h (a, b) or 48 h (c, d). Anti-Erg (red) labels EC nuclei. EdU labels the nuclei of all cells in S-phase in the previous 4 h. Blue nuclei mark non-endothelial cells in S-phase, and double-positive (Erg+/EdU+) cell nuclei are pseudocoloured green to better highlight ECs in S-phase. Scale bars, 200 μm.
RLCs selectively differentiate to intraglomerular mesangial cells during EC model.
Panel A shown only. Representative confocal microscopy images for day 0 and day 7 of β-gal and the EC markers ERG (upper panels) or CD31 (lower panels) co-stained kidney slices.
Immunostainings and Acid fuchsin orange G (AFOG) staining were performed on paraffin-embedded 4-μm kidney sections. Antibodies were diluted in 1% BSA/TBS. Primary antibodies were incubated overnight at 4°C, secondary antibodies and Avidin D-conjugated horseradish peroxidase were incubated for 2 hours at room temperature. All immunofluorescence samples were counterstained with the nuclear marker 4′,6-diamidino-2-phenylindole (DAPI) and mounted with mowiol mounting medium. Endogenous peroxidase activity was suppressed with 3% hydrogen peroxide solution. Samples were counterstained with hematoxylin, dehydrated and mounted. The following antibodies were used: WT-1 (ab202639), nephrin, PDGFRβ (ab91066), α8-integrin, CD31, ERG (ab196149), β-galactosidase (ab9361), renin, CD45 (ab64100).
Mt4-mmp expression during early mouse embryonic development.
Panel K shown only: Double-labeled cells for the endothelial markers ERG (red, arrows in K) and β-gal (green) demonstrate that cells expressing Mt4-mmp in this location are endothelial cells. Abbreviations: da, dorsal aorta; fp, floor plate; s, somite; NCC, neural crest cells; NT, neural tube. Scale bars: 30 μm (J-L).
For immunohistochemical procedures, embryos were fixed in 4% PFA in PBS 0.1M pH 7.2 by immersion or perfusion depending on the developmental stage, cryoprotected in a 30% sucrose solution, and then embedded in OCT and sectioned in the cryostat at 20 μm thickness in the transverse plane. Immunohistochemistry was performed following standard protocols. Primary antibodies used include: polyclonal anti-CD31 hamster (1/1000), anti-β-galactosidase rabbit (1/1000; ab4761, Abcam), anti-FoxA2 mouse (1/250), anti-Nkx6.1 mouse (1/1000), anti-Olig2 rabbit (1/1000), anti-ERG-647 rabbit (1/500; ab196149, Abcam) and anti-WT-1 mouse (Wilms Tumor-1; 1/50;). Sections were incubated with the primary antibody diluted in PBS containing 0.1% Triton X-100 and 1% bovine serum albumin (BSA), for 48 h at 4°C. Subsequently, the sections were rinsed in PBS and incubated for 2 hours at room temperature with 488 or 594-Alexa™-conjugated fluorescent antibodies (1/1000). Sections were counterstained with Hoechst (1:1000) for 5 min at room temperature to visualize nuclei.
ab196149 staining ERG in THP-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196149 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab196149 has been referenced in 9 publications.
- Esteban S et al. Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis. EMBO Mol Med 12:e10862 (2020). PubMed: 31793743
- Poulet M et al. PRL-2 phosphatase is required for vascular morphogenesis and angiogenic signaling. Commun Biol 3:603 (2020). PubMed: 33097786
- Brulois K et al. A molecular map of murine lymph node blood vascular endothelium at single cell resolution. Nat Commun 11:3798 (2020). PubMed: 32732867
- Alonso-Herranz L et al. Macrophages promote endothelial-to-mesenchymal transition via MT1-MMP/TGFß1 after myocardial infarction. Elife 9:N/A (2020). PubMed: 33063665
- Yanagida K et al. Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization. Dev Cell 52:779-793.e7 (2020). PubMed: 32059774
- Pontes-Quero S et al. High mitogenic stimulation arrests angiogenesis. Nat Commun 10:2016 (2019). IHC (PFA fixed) ; Mouse . PubMed: 31043605
- Fernández-Chacón M et al. iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications. Nat Commun 10:2262 (2019). IHC (PFA fixed) ; Mouse . PubMed: 31118412
- Ruhnke L et al. Progenitor Renin Lineage Cells are not involved in the regeneration of glomerular endothelial cells during experimental renal thrombotic microangiopathy. PLoS One 13:e0196752 (2018). IHC-P ; Mouse . PubMed: 29771991
- Blanco MJ et al. Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One 12:e0184767 (2017). IHC-FoFr ; Mouse . PubMed: 28926609