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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
  • Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR3864] to ERG
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free Carrier Free HRP Unconjugated

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Overview

  • Product name

    Alexa Fluor® 647 Anti-ERG antibody [EPR3864]
    See all ERG primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR3864] to ERG
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: THP-1 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Dissociation constant (KD)

    KD = 8.90 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3864
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation

Associated products

  • Alternative Versions

    • HRP Anti-ERG antibody [EPR3864] (ab195514)
    • Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)
    • Anti-ERG antibody [EPR3864] - BSA and Azide free (ab214796)
    • Anti-ERG antibody [EPR3864] (ab92513)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ERG protein (ab185597)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)

Notes
ICC/IF
1/100.

This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
  • Involvement in disease

    Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
    Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P11308 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2078 Human
    • Entrez Gene: 13876 Mouse
    • Entrez Gene: 170909 Rat
    • Omim: 165080 Human
    • SwissProt: P11308 Human
    • SwissProt: P81270 Mouse
    • Unigene: 473819 Human
    • Unigene: 164531 Mouse
    • Alternative names

      • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
      • D030036I24Rik antibody
      • Erg 3 antibody
      • Erg antibody
      • ERG/EWS fusion gene, included antibody
      • ERG/FUS fusion gene, included antibody
      • ERG/TMPSSR2 fusion gene, included antibody
      • ERG_HUMAN antibody
      • ERG1, included antibody
      • ERG2, included antibody
      • ets related antibody
      • ETS-related gene antibody
      • KCNH2 antibody
      • Oncogene ERG antibody
      • p55 antibody
      • TMPRSS2/ERG fusion antibody
      • transcriptional regulator ERG (transforming protein ERG) antibody
      • Transcriptional regulator ERG antibody
      • Transforming protein ERG antibody
      • v ets avian erythroblastosis virus E26 oncogene antibody
      • v ets avian erythroblastosis virus E26 oncogene related antibody
      • v ets erythroblastosis virus E26 oncogene homolog antibody
      • v ets erythroblastosis virus E26 oncogene like antibody
      • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
      • v-ets erythroblastosis virus E26 oncogene antibody
      • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
      • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)Pontes-Quero S et al Nat Commun. 2019 May 1;10(1):2016. doi: 10.1038/s41467-019-09875-7. Fig 2a. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      For mouse retina immunostaining, eyes were collected at the indicated time points and fixed in 4% PFA in PBS for 1 h at room temperature (RT). After two PBS washes, retinas were micro-dissected and stained. Briefly, retinas were blocked and permeabilized with 0.3% Triton X-100, 3% fetal bovine serum (FBS) and 3% donkey serum in PBS. Samples were then washed twice in PBLEC buffer (1 mM CaCl2, 1 mM MgCl2, 1 mM MnCl2 and 1% Triton X-100 in PBS). Biotinylated isolectinB4 or primary antibodies (Panel a, ab196149 1/100 dilution) were diluted in PBLEC buffer and tissues were incubated in this solution for 2 h at RT or overnight at 4 °C. After five washes in blocking solution diluted 1:2, samples were incubated for 1 h at RT with Alexa-conjugated secondary antibody. After two washes in PBS, retinas were mounted with Fluoromount-G. To detect EdU-labeled DNA, an additional step was performed before mounting using the Click-It EdU kit.

      Dll4/Notch signalling inhibition induces context-dependent proliferative effects. a–d Confocal micrographs of the postnatal retinal vasculature from animals treated at P5 with IgG (control) or anti-Dll4 for 24 h (a, b) or 48 h (c, d). Anti-Erg (red) labels EC nuclei. EdU labels the nuclei of all cells in S-phase in the previous 4 h. Blue nuclei mark non-endothelial cells in S-phase, and double-positive (Erg+/EdU+) cell nuclei are pseudocoloured green to better highlight ECs in S-phase. Scale bars, 200 μm.

       

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)Ruhnke L et al PLoS One. 2018 May 17;13(5):e0196752. doi: 10.1371/journal.pone.0196752. eCollection 2018. Fig 3a. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      RLCs selectively differentiate to intraglomerular mesangial cells during EC model.
      Panel A shown only. Representative confocal microscopy images for day 0 and day 7 of β-gal and the EC markers ERG (upper panels) or CD31 (lower panels) co-stained kidney slices.

      Immunostainings and Acid fuchsin orange G (AFOG) staining were performed on paraffin-embedded 4-μm kidney sections. Antibodies were diluted in 1% BSA/TBS. Primary antibodies were incubated overnight at 4°C, secondary antibodies and Avidin D-conjugated horseradish peroxidase were incubated for 2 hours at room temperature. All immunofluorescence samples were counterstained with the nuclear marker 4′,6-diamidino-2-phenylindole (DAPI) and mounted with mowiol mounting medium. Endogenous peroxidase activity was suppressed with 3% hydrogen peroxide solution. Samples were counterstained with hematoxylin, dehydrated and mounted. The following antibodies were used: WT-1 (ab202639), nephrin, PDGFRβ (ab91066), α8-integrin, CD31, ERG (ab196149), β-galactosidase (ab9361), renin, CD45 (ab64100).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)Blanco MJ et al PLoS One. 2017 Sep 19;12(9):e0184767. doi: 10.1371/journal.pone.0184767. eCollection 2017. Fig 1k. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Mt4-mmp expression during early mouse embryonic development.


      Panel K shown only: Double-labeled cells for the endothelial markers ERG (red, arrows in K) and β-gal (green) demonstrate that cells expressing Mt4-mmp in this location are endothelial cells. Abbreviations: da, dorsal aorta; fp, floor plate; s, somite; NCC, neural crest cells; NT, neural tube. Scale bars: 30 μm (J-L).

      For immunohistochemical procedures, embryos were fixed in 4% PFA in PBS 0.1M pH 7.2 by immersion or perfusion depending on the developmental stage, cryoprotected in a 30% sucrose solution, and then embedded in OCT and sectioned in the cryostat at 20 μm thickness in the transverse plane. Immunohistochemistry was performed following standard protocols. Primary antibodies used include: polyclonal anti-CD31 hamster (1/1000), anti-β-galactosidase rabbit (1/1000; ab4761, Abcam), anti-FoxA2 mouse (1/250), anti-Nkx6.1 mouse (1/1000), anti-Olig2 rabbit (1/1000), anti-ERG-647 rabbit (1/500; ab196149, Abcam) and anti-WT-1 mouse (Wilms Tumor-1; 1/50;). Sections were incubated with the primary antibody diluted in PBS containing 0.1% Triton X-100 and 1% bovine serum albumin (BSA), for 48 h at 4°C. Subsequently, the sections were rinsed in PBS and incubated for 2 hours at room temperature with 488 or 594-Alexa™-conjugated fluorescent antibodies (1/1000). Sections were counterstained with Hoechst (1:1000) for 5 min at room temperature to visualize nuclei.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)

      ab196149 staining ERG in THP-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196149 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
      Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (9)

    Publishing research using ab196149? Please let us know so that we can cite the reference in this datasheet.

    ab196149 has been referenced in 9 publications.

    • Esteban S  et al. Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis. EMBO Mol Med 12:e10862 (2020). PubMed: 31793743
    • Poulet M  et al. PRL-2 phosphatase is required for vascular morphogenesis and angiogenic signaling. Commun Biol 3:603 (2020). PubMed: 33097786
    • Brulois K  et al. A molecular map of murine lymph node blood vascular endothelium at single cell resolution. Nat Commun 11:3798 (2020). PubMed: 32732867
    • Alonso-Herranz L  et al. Macrophages promote endothelial-to-mesenchymal transition via MT1-MMP/TGFß1 after myocardial infarction. Elife 9:N/A (2020). PubMed: 33063665
    • Yanagida K  et al. Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization. Dev Cell 52:779-793.e7 (2020). PubMed: 32059774
    • Pontes-Quero S  et al. High mitogenic stimulation arrests angiogenesis. Nat Commun 10:2016 (2019). IHC (PFA fixed) ; Mouse . PubMed: 31043605
    • Fernández-Chacón M  et al. iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications. Nat Commun 10:2262 (2019). IHC (PFA fixed) ; Mouse . PubMed: 31118412
    • Ruhnke L  et al. Progenitor Renin Lineage Cells are not involved in the regeneration of glomerular endothelial cells during experimental renal thrombotic microangiopathy. PLoS One 13:e0196752 (2018). IHC-P ; Mouse . PubMed: 29771991
    • Blanco MJ  et al. Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One 12:e0184767 (2017). IHC-FoFr ; Mouse . PubMed: 28926609

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-ERG antibody [EPR3864] (Alexa Fluor® 647)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (White adipose tissue)
    Specification
    White adipose tissue
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 13 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-ERG antibody [EPR3864] (Alexa Fluor® 647)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Peripheral lymph node)
    Permeabilization
    Yes - 0.05% Tween, 0.1% Triton
    Specification
    Peripheral lymph node
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Agata Szade

    Verified customer

    Submitted Dec 06 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ERG antibody [EPR3864] (Alexa Fluor® 647)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.5% Triton-X
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 26°C
    Fixative
    Zink
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 17 2016

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