Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)

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Flow Cytometry - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)
  • Flow Cytometry - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)
  • Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [E460] to ERK2
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-ERK2 antibody [E460]
    See all ERK2 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [E460] to ERK2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab190581 reacts with an epitope located in the C terminal region of ERK2.

  • Positive control

    • Flow Cyt: HeLa cells.HAP1-WT cells.

  • General notes

    Alternative versions available:
    Anti-ERK2 antibody [E460] (ab32081) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
All applications
Mouse
Rat
Recombinant fragment
Application Abreviews Notes
Flow Cyt
1/500.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

 
Notes
Flow Cyt
1/500.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.

  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

  • Post-translational
    modifications

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ERK 2 antibody
    • ERK-2 antibody
    • ERT1 antibody
    • Extracellular Signal Regulated Kinase 2 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase isoform p42 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • MK01_HUMAN antibody
    • P38 antibody
    • P40 antibody
    • P41 antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • protein kinase, mitogen-activated, 1 antibody
    • protein kinase, mitogen-activated, 2 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

Images

  • Flow Cytometry - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)
    Flow Cytometry - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-MAPK1 knockout cells (red line) stained with ab190581. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab190581, 0.01µg/ml dilution) for 30 min at 22°C.

    A rabbit monoclonal IgG isotype control antibody  (ab199093) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-MAPK1   knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • Flow Cytometry - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)
    Flow Cytometry - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)

    Overlay histogram showing HeLa cells stained with ab190581 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab190581, 1/500 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)
    Alexa Fluor® 647 Anti-ERK2 antibody [E460] (ab190581)

References (0)

Publishing research using ab190581? Please let us know so that we can cite the reference in this datasheet.

ab190581 has not yet been referenced specifically in any publications.

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