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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box FOXA
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)
  • Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR10881] to FOXA1
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Primary
Product image
Anti-FOXA1 antibody [EPR10881] - BSA and Azide free (ab236011)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881]
    See all FOXA1 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR10881] to FOXA1
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Positive control

    • ICC/IF: HepG2 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10881
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXA
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-FOXA1 antibody [EPR10881] - ChIP Grade (ab170933)
    • Anti-FOXA1 antibody [EPR10881-14] (ab173287)
    • Alexa Fluor® 488 Anti-FOXA1 antibody [EPR10881] (ab197235)
    • Anti-FOXA1 antibody [EPR10881] - BSA and Azide free (ab236011)
    • Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
Application Abreviews Notes
ICC/IF
1/100.
Notes
ICC/IF
1/100.

Target

  • Function

    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • Tissue specificity

    Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P55317 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3169 Human
    • Omim: 602294 Human
    • SwissProt: P55317 Human
    • Unigene: 163484 Human
    • Alternative names

      • forkhead box A1 antibody
      • Forkhead box protein A1 antibody
      • FOX A1 antibody
      • FOXA1 antibody
      • FOXA1_HUMAN antibody
      • hepatocyte nuclear factor 3 alpha antibody
      • Hepatocyte nuclear factor 3-alpha antibody
      • HNF 3A antibody
      • HNF-3-alpha antibody
      • HNF-3A antibody
      • HNF3A antibody
      • MGC33105 antibody
      • TCF 3A antibody
      • TCF-3A antibody
      • TCF3A antibody
      • Transcription factor 3A antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)

      ab196908 staining FOXA1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196908 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)
      Alexa Fluor® 647 Anti-FOXA1 antibody [EPR10881] (ab196908)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab196908? Please let us know so that we can cite the reference in this datasheet.

    ab196908 has not yet been referenced specifically in any publications.

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