Recombinant Alexa Fluor® 647 Anti-FOXG1 antibody [EPR18987] (ab270103)
![Immunocytochemistry - Alexa Fluor® 647 Anti-FOXG1 antibody [EPR18987] (ab270103)](https://www.abcam.com/ps/products/270/ab270103/Images/ab270103-415165-ICCAP00455091jpeg.jpg "Immunocytochemistry - Alexa Fluor® 647 Anti-FOXG1 antibody [EPR18987] (ab270103)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR18987] to FOXG1
- Suitable for: ICC
- Reacts with: Rat
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-FOXG1 antibody [EPR18987]
See all FOXG1 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR18987] to FOXG1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide within Human FOXG1 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: P55316 -
Positive control
- ICC: C6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 68.98% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18987 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
Our Abpromise guarantee covers the use of ab270103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/50. This product gave a positive signal in C6 fixed with 4% formaldehyde (10 min). |
Target
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Function
Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. -
Tissue specificity
Expression is restricted to the neurons of the developing telencephalon. -
Involvement in disease
Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 24370 Rat
- SwissProt: Q00939 Rat
- Unigene: 9864 Rat
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Alternative names
- BF-1 antibody
- BF-2 antibody
- BF1 antibody
see all
Images
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Immunocytochemistry - Alexa Fluor® 647 Anti-FOXG1 antibody [EPR18987] (ab270103)
Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling FOXG1 with ab270103. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270103 at 1/50 dilution (Red) and ab195887, mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (Green). Nuclear DNA was labelled with DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab270103 has not yet been referenced specifically in any publications.
