Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)](https://www.abcam.com/ps/products/191/ab191114/Images/ab191114-343285-ab191114.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [TU-30] to gamma Tubulin - C-terminal
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Plants
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal
See all gamma Tubulin primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [TU-30] to gamma Tubulin - C-terminal -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Specificity
Immunogen has 94% identity with TUBG2 (Uniprot: Q9NRH3). -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Cow, Human, Pig, Plants -
Immunogen
Synthetic peptide corresponding to Human gamma Tubulin aa 434-449 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:EYHAATRPDYISWGTQ
Database link: P23258 -
General notes
Also reacts with Protozoa.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
TU-30 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab191114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsPhosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 540216 Cow
- Entrez Gene: 541101 Cow
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
see all -
Alternative names
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)
Immunocytochemistry/ Immunofluorescence analysis of P19X1 mouse embryonal carcinoma cell line labeling gamma Tubulin with ab191114 at 1 μg/mL.
Nuclei were stained with DAPI (blue).
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![Flow Cytometry - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)](https://www.abcam.com/ps/products/191/ab191114/Images/ab191114-343737-antigammatubulinantibody-TU30-Cterminal-Alexa-Fluor-647-flowcytometry-human-jurkat.jpg)
Flow Cytometry - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)Flow Cytometry analysis of Jurkat cells labeling gamma Tubulin with ab191114.
Protocols
References (2)
ab191114 has been referenced in 2 publications.
- Rashid R et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data 6:323 (2019). beta actin ; Human . PubMed: 31848351
- Kiseleva AA et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs. Clin Cancer Res 25:4179-4193 (2019). PubMed: 30867219
