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Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [3E8AD9] to GAPDH
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2b

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    Overview

    • Product name

      Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9]
      See all GAPDH primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [3E8AD9] to GAPDH
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      Tissue, cells or virus corresponding to Human GAPDH. Purified protein from Human erythrocytes.

    • Positive control

      • ICC/IF: HeLa cells.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 1% BSA, PBS, 30% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purification notes

      ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE.
    • Clonality

      Monoclonal
    • Clone number

      3E8AD9
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Huntington's disease
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Neurodegenerative disease

    Associated products

    • Alternative Versions

      • Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
      • Alexa Fluor® 488 Anti-GAPDH antibody [3E8AD9] (ab198305)
      • HRP Anti-GAPDH antibody [3E8AD9] - Loading Control (ab198306)
    • Positive Controls

      • Hep G2 cytoplasmic extract lysate (ab14659)
    • Recombinant Protein

      • Recombinant human GAPDH protein (Active) (ab82633)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab198105 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    Application Abreviews Notes
    ICC/IF
    1/100.

    This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Notes
    ICC/IF
    1/100.

    This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

    Target

    • Function

      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similarities

      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications

      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Target information above from: UniProt accession P04406 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2597 Human
      • Omim: 138400 Human
      • SwissProt: P04406 Human
      • Unigene: 544577 Human
      • Unigene: 592355 Human
      • Unigene: 598320 Human
      • Alternative names

        • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
        • aging associated gene 9 protein antibody
        • Aging-associated gene 9 protein antibody
        • BARS-38 antibody
        • cb609 antibody
        • EC 1.2.1.12 antibody
        • Epididymis secretory sperm binding protein Li 162eP antibody
        • G3P_HUMAN antibody
        • G3PD antibody
        • G3PDH antibody
        • GAPD antibody
        • GAPDH antibody
        • Glyceraldehyde 3 phosphate dehydrogenase antibody
        • glyceraldehyde 3-PDH antibody
        • Glyceraldehyde-3-phosphate dehydrogenase antibody
        • HEL-S-162eP antibody
        • KNC-NDS6 antibody
        • MGC102544 antibody
        • MGC102546 antibody
        • MGC103190 antibody
        • MGC103191 antibody
        • MGC105239 antibody
        • MGC127711 antibody
        • MGC88685 antibody
        • OCAS, p38 component antibody
        • OCT1 coactivator in S phase, 38-KD component antibody
        • peptidyl cysteine S nitrosylase GAPDH antibody
        • Peptidyl-cysteine S-nitrosylase GAPDH antibody
        • wu:fb33a10 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [3E8AD9] (ab198105)

        ab198105 staining GAPDH in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198105 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab198105? Please let us know so that we can cite the reference in this datasheet.

      ab198105 has not yet been referenced specifically in any publications.

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