Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [mAbcam 9484] to GAPDH
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG2b

    Overview

    • Product name

      Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484]
      See all GAPDH primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [mAbcam 9484] to GAPDH

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Cynomolgus monkey, Chinese hamster

    • Immunogen

      Full length native protein (purified) corresponding to Human GAPDH.

    • Positive control

      • ICC/IF: HeLa cells.

    • General notes

       

       

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab196697 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/100.
    Notes
    ICC/IF
    1/100.

    Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)

      ab196697 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196697 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    References (0)

    Publishing research using ab196697? Please let us know so that we can cite the reference in this datasheet.

    ab196697 has not yet been referenced specifically in any publications.

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