RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
  • Flow Cytometry - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
  • Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker
    See all GM130 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP892Y] to GM130 - cis-Golgi Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A synthetic peptide corresponding to residues near the N-term of human GM130.

  • Positive control

    • ICC/IF - HeLa, Flow Cyt - HeLa

  • General notes

             

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab195303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µl for 106 cells.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Function

    Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.

  • Sequence similarities

    Belongs to the GOLGA2 family.

  • Domain

    Extended rod-like protein with coiled-coil domains.

  • Cellular localization

    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 130 kDa cis Golgi matrix protein antibody
      • 130 kDa cis-Golgi matrix protein antibody
      • Cis golgi matrix protein GM130 antibody
      • GM130 antibody
      • Gm130 autoantigen antibody
      • GOGA2_HUMAN antibody
      • GOLGA 2 antibody
      • Golga2 antibody
      • Golgi autoantigen antibody
      • Golgi autoantigen golgin subfamily a 2 antibody
      • Golgi matrix protein GM130 antibody
      • Golgin 95 antibody
      • golgin A2 antibody
      • Golgin subfamily a 2 antibody
      • Golgin subfamily A member 2 antibody
      • Golgin-95 antibody
      • MGC20672 antibody
      • SY11 protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

      Ab195303 staining GM130 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195303 at 5μg/ml (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1/100 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      This product gave a positive signal in 4% formaldehyde (10min) fixed HeLa cells under the same testing conditions.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
      Flow Cytometry - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

      Overlay histogram showing HeLa cells stained with ab195303 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195303, 0.1μg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Alexa® 647) (0.2μl/1x106 cells) for 30 min at 22ºC. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 25mW red solid state diode laser (635nm) and 675/30 bandpass filter.

      This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
      Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

    References (1)

    Publishing research using ab195303? Please let us know so that we can cite the reference in this datasheet.

    ab195303 has been referenced in 1 publication.

    • Odhams CA  et al. Interferon inducible X-linked gene CXorf21 may contribute to sexual dimorphism in Systemic Lupus Erythematosus. Nat Commun 10:2164 (2019). PubMed: 31092820

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (Alexa Fluor® 647)

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Jul 22 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (Alexa Fluor® 647)

     Average
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Chinese Hamster Cell (chinese hamster ovary cell)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    chinese hamster ovary cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 29 2015

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