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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation Lysine demethylation
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)

  • Datasheet
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
  • Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP Unconjugated

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Knockout
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Human KDM1A (LSD1) knockout HeLa cell line (ab265790)
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Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker
    See all KDM1/LSD1 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab166919)

  • Positive control

    • ICC/IF: HeLa cells, HAP1-KDM1A cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6825
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195)
    • Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
    • HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
    • Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
  • Immunizing Peptide (Blocking)

    • KDM1/LSD1 peptide (ab166919)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/500.
Notes
ICC/IF
1/100 - 1/500.

Target

  • Function

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O60341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23028 Human
    • Entrez Gene: 99982 Mouse
    • Entrez Gene: 500569 Rat
    • Omim: 609132 Human
    • SwissProt: O60341 Human
    • SwissProt: Q6ZQ88 Mouse
    • Unigene: 591518 Human
    • Unigene: 28540 Mouse
    • Alternative names

      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)

      ab195305 staining KDM1A in wild-type HAP1 cells (top panel) and KDM1A knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195305 at 1/500 dilution (shown in red) and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)

      ab195305 staining KDM1 / LSD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195305 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
      Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab195305? Please let us know so that we can cite the reference in this datasheet.

    ab195305 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Flow Cytometry abreview for Anti-KDM1/LSD1 antibody [EPR6825] (Alexa Fluor® 647) - Nuclear Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Embryonic stem cells)
    Permeabilization
    Yes - Methanol
    Gating Strategy
    ESC cultured in differentiation medium
    Specification
    Embryonic stem cells
    Preparation
    Cell harvesting/tissue preparation method: cells were dissociated by using trypLE
    Sample buffer: 0.5%BSA in PBS
    Fixation
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Rujuan Zuo

    Verified customer

    Submitted Aug 12 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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