Recombinant Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)](https://www.abcam.com/ps/products/199/ab199818/Images/ab199818-252861-ICCIFAP00247101.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP1974Y] to MCM7/PRL
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y]
See all MCM7/PRL primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP1974Y] to MCM7/PRL -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human MCM7/PRL aa 650 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- ICC/IF: HeLa cells
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General notes
This product was previously labelled as MCM7
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1974Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab199818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4176 Human
- Entrez Gene: 17220 Mouse
- Entrez Gene: 288532 Rat
- Omim: 600592 Human
- SwissProt: P33993 Human
- SwissProt: Q61881 Mouse
- Unigene: 438720 Human
- Unigene: 378965 Mouse
see all -
Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
ab199818 staining MCM7/PRL in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199818 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)](https://www.abcam.com/ps/products/199/ab199818/Images/ab199818-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab199818 has not yet been referenced specifically in any publications.
