Recombinant Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)](https://www.abcam.com/ps/products/199/ab199818/Images/ab199818-252861-ICCIFAP00247101.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP1974Y] to MCM7/PRL
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y]
See all MCM7/PRL primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP1974Y] to MCM7/PRL -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells
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General notes
This product was previously labelled as MCM7
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1974Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
| Notes |
|---|
|
ICC/IF
1/50. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4176 Human
- Entrez Gene: 17220 Mouse
- Entrez Gene: 288532 Rat
- Omim: 600592 Human
- SwissProt: P33993 Human
- SwissProt: Q61881 Mouse
- Unigene: 438720 Human
- Unigene: 378965 Mouse
see all -
Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
ab199818 staining MCM7/PRL in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199818 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)](https://www.abcam.com/ps/products/199/ab199818/Images/ab199818-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab199818 has not yet been referenced specifically in any publications.