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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
  • Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP1974Y] to MCM7/PRL
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Protein
Product image
Recombinant Human MCM7/PRL protein (ab114356)
Conjugation
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y]
    See all MCM7/PRL primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP1974Y] to MCM7/PRL
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as MCM7

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP1974Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-MCM7/PRL antibody [EP1974Y] (ab199753)
    • Anti-MCM7/PRL antibody [EP1974Y] - BSA and Azide free (ab242382)
    • Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab280970)
    • Alexa Fluor® 488 Anti-MCM7/PRL antibody [EP1974Y] (ab280971)
    • Anti-MCM7/PRL antibody [EP1974Y] (ab52489)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant Human MCM7/PRL protein (ab114356)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/50.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Notes
ICC/IF
1/50.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • Sequence similarities

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P33993 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4176 Human
    • Entrez Gene: 17220 Mouse
    • Entrez Gene: 288532 Rat
    • Omim: 600592 Human
    • SwissProt: P33993 Human
    • SwissProt: Q61881 Mouse
    • Unigene: 438720 Human
    • Unigene: 378965 Mouse
    • Unigene: 113 Rat
    see all
  • Alternative names

    • CDABP0042 antibody
    • CDC 47 antibody
    • CDC47 antibody
    • CDC47 homolog antibody
    • Cdc47, S. cerevisiae, homolog of antibody
    • DNA replication licensing factor MCM7 antibody
    • Homolog of S. cerevisiae Cdc47 antibody
    • MCM 2 antibody
    • MCM 7 antibody
    • MCM2 antibody
    • MCM2, formerly antibody
    • Mcm7 antibody
    • MCM7 minichromosome maintenance deficient 7 antibody
    • MCM7_HUMAN antibody
    • Minichromosome Maintainence 7 antibody
    • Minichromosome maintainence, S. cerevisiae, homolog of antibody
    • Minichromosome maintenance complex component 7 antibody
    • Minichromosome maintenance deficient 7 antibody
    • Minichromosome maintenance protein 7 antibody
    • P1.1 MCM3 antibody
    • P1.1-MCM3 antibody
    • P1CDC47 antibody
    • P85MCM antibody
    • PNAS 146 antibody
    • PNAS146 antibody
    • PPP1R104 antibody
    • Protein phosphatase 1, regulatory subunit 104 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)

    ab199818 staining MCM7/PRL in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199818 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)
    Alexa Fluor® 647 Anti-MCM7/PRL antibody [EP1974Y] (ab199818)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab199818? Please let us know so that we can cite the reference in this datasheet.

ab199818 has not yet been referenced specifically in any publications.

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