Recombinant Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)](https://www.abcam.com/ps/products/204/ab204478/Images/ab204478-248124-ab204478-ap0022998-HeLap-5ug2.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR10879] to MKLP1
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879]
See all MKLP1 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR10879] to MKLP1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MKLP1 aa 800-900 (Cysteine residue). The exact sequence is proprietary.
Database link: Q02241 -
Positive control
- ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10879 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
| Notes |
|---|
|
ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Target
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Function
Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles. -
Sequence similarities
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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Database links
- Entrez Gene: 9493 Human
- Omim: 605064 Human
- SwissProt: Q02241 Human
- Unigene: 270845 Human
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Alternative names
- CHO 1 antibody
- CHO1 antibody
- KIF 23 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)
ab204478 staining MKLP1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204478 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)](https://www.abcam.com/ps/products/204/ab204478/Images/ab204478-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab204478 has been referenced in 2 publications.
- Liu X et al. Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly. J Mol Cell Biol N/A:N/A (2019). PubMed: 31174204
- Li Y et al. microRNA-300/NAMPT regulates inflammatory responses through activation of AMPK/mTOR signaling pathway in neonatal sepsis. Biomed Pharmacother 108:271-279 (2018). PubMed: 30223098