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Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

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Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

Key features and details

  • Alexa Fluor® 647 Mouse monoclonal [9E10] to Myc tag
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Recombinant fragment
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 APC Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC HRP PE SureLight® APC Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-Myc tag antibody [9E10]
    See all Myc tag primary antibodies

  • Description

    Alexa Fluor® 647 Mouse monoclonal [9E10] to Myc tag

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Specificity

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    aa 410-419 of human Myc.

  • Positive control

    • ICC/IF: 293T cells transfected with TMEM119 with myc tag Flow Cyt: 293T cells transfected with TMEM119 with myc tag

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    9E10

  • Myeloma

    Sp2/0

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in 293T cells transfected with TMEM119 with myc tag fixed with 4% formaldehyde (10 min).
Flow Cyt
1/500.
Notes
ICC/IF
1/100.

This product gave a positive signal in 293T cells transfected with TMEM119 with myc tag fixed with 4% formaldehyde (10 min).
Flow Cyt
1/500.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • Cellular localization

    Nuclear

  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

Images

  • Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
    Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab223895 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Alexa Fluor® 647 (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody (ab223895) and Alexa Fluor® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)

    ab223895 staining Myc-tag (pseudocolored in white) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223895 at 1/100 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor® 488 conjugated control Myc-tag antibody (different clone) is shown in green.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab223895? Please let us know so that we can cite the reference in this datasheet.

ab223895 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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