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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)
  • Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [UOTR1B492] to NADPH oxidase 4
  • Suitable for: ICC/IF
  • Reacts with: Rat, Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Primary
Product image
Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab109225)
Protein
Product image
Recombinant Human NADPH oxidase 4 protein (ab112414)
Primary
Product image
Alexa Fluor® 488 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195300)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492]
    See all NADPH oxidase 4 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [UOTR1B492] to NADPH oxidase 4
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NADPH oxidase 4 aa 500 to the C-terminus.
    (Peptide available as ab179799)

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    UOTR1B492
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab109225)
    • Alexa Fluor® 488 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195300)
    • HRP Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195524)
    • Anti-NADPH oxidase 4 antibody [UOTR1B492] - BSA and Azide free (ab230322)
  • Immunizing Peptide (Blocking)

    • NADPH oxidase 4 peptide (ab179799)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Positive Controls

    • U-87 MG nuclear extract lysate (ab14903)
  • Recombinant Protein

    • Recombinant Human NADPH oxidase 4 protein (ab112414)

Applications

Our Abpromise guarantee covers the use of ab195301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/35.

Target

  • Function

    Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.
  • Tissue specificity

    Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
  • Sequence similarities

    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Developmental stage

    Expressed in fetal kidney and fetal liver.
  • Post-translational
    modifications

    Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
  • Cellular localization

    Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
  • Target information above from: UniProt accession Q9NPH5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50507 Human
    • Entrez Gene: 85431 Rat
    • Omim: 605261 Human
    • SwissProt: Q9NPH5 Human
    • SwissProt: Q924V1 Rat
    • Unigene: 371036 Human
    • Unigene: 14744 Rat
    • Alternative names

      • Kidney oxidase 1 antibody
      • Kidney oxidase-1 antibody
      • Kidney superoxide producing NADPH oxidase antibody
      • Kidney superoxide-producing NADPH oxidase antibody
      • KOX 1 antibody
      • KOX antibody
      • Kox-1 antibody
      • KOX1 antibody
      • NADPH antibody
      • NADPH oxidase 4 antibody
      • Nox4 antibody
      • NOX4_HUMAN antibody
      • Renal NAD(P)H oxidase antibody
      • Renal NAD(P)H-oxidase antibody
      • RENOX antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

      ab195301 staining NADPH oxidase 4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195301 at 1/35 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      This product also gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)
      Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab195301? Please let us know so that we can cite the reference in this datasheet.

    ab195301 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab195301.
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