RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)
  • Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [UOTR1B492] to NADPH oxidase 4
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492]
    See all NADPH oxidase 4 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [UOTR1B492] to NADPH oxidase 4

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Dog

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab179799)

  • Positive control

    • ICC/IF: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/35.
Notes
ICC/IF
1/35.

Target

  • Function

    Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.

  • Tissue specificity

    Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

  • Sequence similarities

    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.

  • Developmental stage

    Expressed in fetal kidney and fetal liver.

  • Post-translational
    modifications

    Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

  • Cellular localization

    Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Kidney oxidase 1 antibody
      • Kidney oxidase-1 antibody
      • Kidney superoxide producing NADPH oxidase antibody
      • Kidney superoxide-producing NADPH oxidase antibody
      • KOX 1 antibody
      • KOX antibody
      • Kox-1 antibody
      • KOX1 antibody
      • NADPH antibody
      • NADPH oxidase 4 antibody
      • Nox4 antibody
      • NOX4_HUMAN antibody
      • Renal NAD(P)H oxidase antibody
      • Renal NAD(P)H-oxidase antibody
      • RENOX antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

      ab195301 staining NADPH oxidase 4 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195301 at 1/35 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      This product also gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)
      Alexa Fluor® 647 Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab195301)

    References (0)

    Publishing research using ab195301? Please let us know so that we can cite the reference in this datasheet.

    ab195301 has not yet been referenced specifically in any publications.

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