Alexa Fluor® 647 Anti-NDUFS3 antibody [3F9DD2] (ab197970)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFS3 antibody [3F9DD2] (ab197970)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [3F9DD2] to NDUFS3
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    Overview

    • Product name

      Alexa Fluor® 647 Anti-NDUFS3 antibody [3F9DD2]
      See all NDUFS3 primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [3F9DD2] to NDUFS3

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data

    • Immunogen

      Tissue, cells or virus corresponding to Human NDUFS3.

    • Positive control

      • ICC/IF: HeLa cells.

    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab197970 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    All applications
    Mouse
    Rat
    Cow
    Zebrafish
    Application Abreviews Notes
    ICC/IF
    1/50.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).
    Notes
    ICC/IF
    1/50.

    This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

    Target

    • Function

      Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    • Sequence similarities

      Belongs to the complex I 30 kDa subunit family.

    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CI 30 antibody
      • CI 30KD antibody
      • CI-30kD antibody
      • Complex I 30KD antibody
      • Complex I 30kDa subunit antibody
      • COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody
      • Complex I-30kD antibody
      • mitochondrial antibody
      • NADH coenzyme Q reductase antibody
      • NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody
      • NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody
      • NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody
      • NADH dehydrogenase ubiquinone 30 kDa subunit antibody
      • NADH-ubiquinone oxidoreductase 30 kDa subunit antibody
      • NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody
      • NDUFS3 antibody
      • NDUS3_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFS3 antibody [3F9DD2] (ab197970)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NDUFS3 antibody [3F9DD2] (ab197970)

      ab197970 staining NDUFS3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197970 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    References (0)

    Publishing research using ab197970? Please let us know so that we can cite the reference in this datasheet.

    ab197970 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab197970.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com