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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [rat-401] to Nestin
    • Suitable for: ICC/IF
    • Reacts with: Rat
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

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    Primary
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    Alexa Fluor® 488 Anti-Nestin antibody [rat-401] (ab197495)
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    Anti-Nestin antibody [7A3] (ab81462)

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    Overview

    • Product name

      Alexa Fluor® 647 Anti-Nestin antibody [rat-401]
      See all Nestin primary antibodies
    • Description

      Alexa Fluor® 647 Mouse monoclonal [rat-401] to Nestin
    • Host species

      Rat
    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Tissue, cells or virus corresponding to Rat Nestin. Homogenized spinal cord tissue from embryonic day 15 (E15) rats.

    • Positive control

      • ICC/IF: PC12 cells
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      rat-401
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Cytoskeletal Proteins
      • Intermediate Filaments
      • Neuroscience
      • Cell Type Marker
      • Neural Stem Cell marker
      • Cardiovascular
      • Angiogenesis
      • Angiogenic Factors
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Stem Cells
      • Lineage Markers
      • Ectoderm
      • Stem Cells
      • Neural Stem Cells
      • Intracellular
      • Cancer
      • Tumor biomarkers
      • Tumor antigens
      • Stem Cells
      • Neural Stem Cells
      • Neuron Restricted Lineage
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Other
      • Developmental Biology
      • Lineage specification
      • Ectoderm

    Associated products

    • Alternative Versions

      • HRP Anti-Nestin antibody [rat-401] (ab196694)
      • Alexa Fluor® 488 Anti-Nestin antibody [rat-401] (ab197495)
      • Anti-Nestin antibody [Rat 401] - BSA and Azide free (ab264111)
      • Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker (ab6142)

    Applications

    Our Abpromise guarantee covers the use of ab196693 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/50.

    Target

    • Function

      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificity

      CNS stem cells.
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Developmental stage

      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications

      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Target information above from: UniProt accession P48681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • SwissProt: P21263 Rat
      • Unigene: 9701 Rat
      • Alternative names

        • ESTM 46 antibody
        • FLJ 21841 antibody
        • FLJ21841 antibody
        • Intermediate filament protein antibody
        • Nbla00170 antibody
        • nes antibody
        • NEST_HUMAN antibody
        • Nestin antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Nestin antibody [rat-401] (ab196693)

        ab196693 staining Nestin in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196693 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab196693? Please let us know so that we can cite the reference in this datasheet.

      ab196693 has been referenced in 1 publication.

      • Jung S  et al. Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits. Autophagy N/A:1-19 (2019). PubMed: 31234698

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Flow Cytometry abreview for Anti-Nestin antibody [401] (Alexa Fluor® 647)

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Fixation
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% saponin
      Sample
      Mouse Cell (bone marrow derived mesenchymal stem cells)
      Specification
      bone marrow derived mesenchymal stem cells
      Gating Strategy
      mesenchymal stem cells
      Preparation
      Cell harvesting/tissue preparation method: 0.05% trypsin-edta
      Sample buffer: 0.5% BSA, 0.05% sodium azide
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 24 2015

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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