RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)
  • Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR18049] to NOTCH4
  • Suitable for: ICC/IF
  • Reacts with: Mouse
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049]
    See all NOTCH4 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR18049] to NOTCH4

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in NIH/3T3 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Notes
ICC/IF
1/1000.

This product gave a positive signal in NIH/3T3 fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.

  • Tissue specificity

    Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.

  • Sequence similarities

    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 28 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.

  • Post-translational
    modifications

    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.

  • Cellular localization

    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ16302 antibody
      • hNotch 4 antibody
      • hNotch4 antibody
      • INT3 antibody
      • Neurogenic locus notch homolog protein 4 antibody
      • NOTC4_HUMAN antibody
      • Notch 4 antibody
      • Notch 4 intracellular domain antibody
      • Notch homolog 4 antibody
      • Notch4 antibody
      • NOTCH4 protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)

      ab225329 staining NOTCH4 in NIH/3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225329 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)
      Alexa Fluor® 647 Anti-NOTCH4 antibody [EPR18049] (ab225329)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab225329? Please let us know so that we can cite the reference in this datasheet.

    ab225329 has not yet been referenced specifically in any publications.

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