Recombinant Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (ab204018)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (ab204018)](https://www.abcam.com/ps/products/204/ab204018/Images/ab204018-247493-ICCIFAP00228791.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (ab204018)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP3976] to NuMA
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-NuMA antibody [EP3976]
See all NuMA primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP3976] to NuMA -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide within NuMA. The exact sequence is proprietary.
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Positive control
- ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3976 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| All applications |
Mouse
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/250.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
| Notes |
|---|
|
ICC/IF
1/250. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
Target
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Function
May be a structural component of the nucleus. -
Cellular localization
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. - Information by UniProt
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Database links
- Entrez Gene: 4926 Human
- Entrez Gene: 101706 Mouse
- Entrez Gene: 308870 Rat
- Omim: 164009 Human
- SwissProt: Q14980 Human
- Unigene: 325978 Human
- Unigene: 27259 Mouse
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Alternative names
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- NMP 22 antibody
- Nuclear matrix protein 22 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (ab204018)
ab204018 staining NuMA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204018 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (ab204018)](https://www.abcam.com/ps/products/204/ab204018/Images/ab204018-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-NuMA antibody [EP3976] (ab204018)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab204018 has not yet been referenced specifically in any publications.
