Recombinant Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)](https://www.abcam.com/ps/products/195/ab195180/Images/ab195180-237416-anti-p75-ngf-receptor-antibody-ep1039y-alexa-fluor-647-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EP1039Y] to p75 NGF Receptor
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y]
See all p75 NGF Receptor primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EP1039Y] to p75 NGF Receptor -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide within Human p75 NGF Receptor aa 350-450. The exact sequence is proprietary.
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Positive control
- ICC/IF: PC12 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 3.25 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP1039Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab195180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. |
Target
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Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 24596 Rat
- SwissProt: P07174 Rat
- Unigene: 10980 Rat
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Alternative names
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
ab195180 staining p75 NGF receptor in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195180 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 4% formaldehyde (10 min) fixed PC12 cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)](https://www.abcam.com/ps/products/195/ab195180/Images/ab195180-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (ab195180)
Protocols
Datasheets and documents
References (4)
ab195180 has been referenced in 4 publications.
- Eng J et al. Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis. Methods Mol Biol 2055:521-562 (2020). PubMed: 31502168
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
- Lin JR et al. Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging. Curr Protoc Chem Biol 8:251-264 (2016). PubMed: 27925668
- Lin JR et al. Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method. Nat Commun 6:8390 (2015). PubMed: 26399630
