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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)

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  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)
  • Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP1058Y] to Pan Trk
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Primary
Product image
Alexa Fluor® 488 Anti-Pan Trk antibody [EP1058Y] (ab194321)
Protein
Product image
Recombinant human TrkA protein (Active) (ab60887)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y]
    See all Pan Trk primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP1058Y] to Pan Trk
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Pan Trk.

  • Positive control

    • ICC/IF: U87MG cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1058Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Anti-Pan Trk antibody [EP1058Y] - BSA and Azide free (ab188825)
    • Alexa Fluor® 488 Anti-Pan Trk antibody [EP1058Y] (ab194321)
    • PE Anti-Pan Trk antibody [EP1058Y] (ab209443)
    • Anti-Pan Trk antibody [EP1058Y] (ab76291)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Recombinant Protein

    • Recombinant human TrkA protein (Active) (ab60887)

Applications

Our Abpromise guarantee covers the use of ab194322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in U87MG cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Relevance

    Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane.
  • Database links

    • Entrez Gene: 4914 Human
    • Entrez Gene: 4915 Human
    • Entrez Gene: 4916 Human
    • Entrez Gene: 18211 Mouse
    • Entrez Gene: 18212 Mouse
    • Entrez Gene: 18213 Mouse
    • Entrez Gene: 25054 Rat
    • Entrez Gene: 29613 Rat
    • Entrez Gene: 59109 Rat
    • Omim: 191315 Human
    • Omim: 191316 Human
    • Omim: 600456 Human
    • SwissProt: P04629 Human
    • SwissProt: Q16288 Human
    • SwissProt: Q16620 Human
    • SwissProt: P15209 Mouse
    • SwissProt: Q3UFB7 Mouse
    • SwissProt: Q6VNS1 Mouse
    • SwissProt: P35739 Rat
    • SwissProt: Q03351 Rat
    • SwissProt: Q63604 Rat
    • SwissProt: Q68G04 Rat
    • Unigene: 406293 Human
    • Unigene: 410969 Human
    • Unigene: 494312 Human
    • Unigene: 33496 Mouse
    • Unigene: 421361 Mouse
    • Unigene: 80682 Mouse
    • Unigene: 11246 Rat
    • Unigene: 39098 Rat
    • Unigene: 9963 Rat
    see all
  • Alternative names

    • BDNF/NT-3 growth factors receptor antibody
    • EML4 antibody
    • ETV6 antibody
    • gp140trk antibody
    • GP145-TrkB antibody
    • GP145-TrkC antibody
    • High affinity nerve growth factor receptor antibody
    • LMNA antibody
    • mammary analogue secretory carcinoma antibody
    • MTC antibody
    • neuroendocrine tumor antibody
    • neurotrophic antibody
    • Neurotrophic tyrosine kinase receptor type 1 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophic tyrosine kinase receptor type 3 antibody
    • NT-3 growth factor receptor antibody
    • NTRK antibody
    • NTRK1 antibody
    • NTRK2 antibody
    • NTRK3 antibody
    • p140-TrkA antibody
    • TPM3 antibody
    • TRK antibody
    • TRK fusion antibody
    • Trk-A antibody
    • Trk-B antibody
    • Trk-C antibody
    • TRK1-transforming tyrosine kinase protein antibody
    • TRKA antibody
    • trka+b+c antibody
    • trka/b/c antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKC antibody
    • TrkC tyrosine kinase antibody
    • tropomyosin receptor kinase antibody
    • Tropomyosin-related kinase A antibody
    • Tropomyosin-related kinase B antibody
    • Tropomyosin-related kinase C antibody
    • Tyrosine kinase receptor A antibody
    • Tyrosine kinase receptor antibody
    • tyrosine kinase receptor B antibody
    • tyrosine kinase receptor C antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)

    ab194322 staining Pan Trk in U87MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab194322 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in U87MG cells fixed with 4% formaldehyde (10 min).

  • Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)
    Alexa Fluor® 647 Anti-Pan Trk antibody [EP1058Y] (ab194322)

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab194322? Please let us know so that we can cite the reference in this datasheet.

    ab194322 has been referenced in 1 publication.

    • Mohd Isa IL  et al. Implantation of hyaluronic acid hydrogel prevents the pain phenotype in a rat model of intervertebral disc injury. Sci Adv 4:eaaq0597 (2018). PubMed: 29632893

    Customer reviews and Q&As

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