RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
  • Flow Cytometry - Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
  • Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [28-8] to PD-L1 - Extracellular domain
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Chinese hamster
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain
    See all PD-L1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [28-8] to PD-L1 - Extracellular domain

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Chinese hamster
    ICC/IF
    Chinese hamster
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
    Database link: Q9NZQ7

  • Positive control

    • ICC/IF: CHO-PDL1 cells Flow Cyt: CHO-PDL1 cells

  • General notes

    Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565)

    Alternative versions available:
    Anti-PD-L1 antibody [28-8] (ab205921) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Chinese hamster
ICC/IF
Chinese hamster
All applications
Human
Application Abreviews Notes
Flow Cyt
1/100.
ICC/IF
1/100.

This product gave a positive signal in CHO-PDL1 cells fixed with 4% formaldehyde (10 min)
Notes
Flow Cyt
1/100.
ICC/IF
1/100.

This product gave a positive signal in CHO-PDL1 cells fixed with 4% formaldehyde (10 min)

Target

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane and Endomembrane system.
  • Information by UniProt

  • Database links

    • Alternative names

      • B7 H antibody
      • B7 H1 antibody
      • B7 homolog 1 antibody
      • B7-H1 antibody
      • B7H antibody
      • B7H1 antibody
      • CD 274 antibody
      • CD274 antibody
      • CD274 antigen antibody
      • CD274 molecule antibody
      • MGC142294 antibody
      • MGC142296 antibody
      • OTTHUMP00000021029 antibody
      • PD L1 antibody
      • PD-L1 antibody
      • PD1L1_HUMAN antibody
      • PDCD1 ligand 1 antibody
      • PDCD1L1 antibody
      • PDCD1LG1 antibody
      • PDL 1 antibody
      • PDL1 antibody
      • Programmed cell death 1 ligand 1 antibody
      • Programmed death ligand 1 antibody
      • RGD1566211 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)

      ab209960 staining PDL1 in CHO-PDL1 cells. The lower panels demonstrate that ab209960 does not cross react with un-transfected CHO cells.

      The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209960 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
      Flow Cytometry - Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)

      Overlay histgram showing CHO (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab209960.

      The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209960, 1/100 dilution) for 30 min at 4ºC.

      Acquisition of >5,000 events were collected using a 17mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

    • Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)
      Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain (ab209960)

    References (4)

    Publishing research using ab209960? Please let us know so that we can cite the reference in this datasheet.

    ab209960 has been referenced in 4 publications.

    • Azadi S  et al. Upregulation of PD-L1 expression in breast cancer cells through the formation of 3D multicellular cancer aggregates under different chemical and mechanical conditions. Biochim Biophys Acta Mol Cell Res 1866:118526 (2019). ICC/IF ; Human . PubMed: 31398408
    • La Fleur L  et al. Expression of scavenger receptor MARCO defines a targetable tumor-associated macrophage subset in non-small cell lung cancer. Int J Cancer N/A:N/A (2018). IHC-P ; Human . PubMed: 29667169
    • Chou CK  et al. A flow-proteometric platform for analyzing protein concentration (FAP): Proof of concept for quantification of PD-L1 protein in cells and tissues. Biosens Bioelectron 117:97-103 (2018). PubMed: 29890396
    • Kulasinghe A  et al. PD-L1 expressing circulating tumour cells in head and neck cancers. BMC Cancer 17:333 (2017). ICC/IF ; Human . PubMed: 28511705

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