Alexa Fluor® 647 Anti-PDHA1 antibody [9H9AF5] (ab197653)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PDHA1 antibody [9H9AF5] (ab197653)

    Key features and details

    • Alexa Fluor® 647 Mouse monoclonal [9H9AF5] to PDHA1
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
    • Isotype: IgG1

    Overview

    • Product name

      Alexa Fluor® 647 Anti-PDHA1 antibody [9H9AF5]
      See all PDHA1 primary antibodies

    • Description

      Alexa Fluor® 647 Mouse monoclonal [9H9AF5] to PDHA1

    • Host species

      Mouse

    • Conjugation

      Alexa Fluor® 647. Ex: 652nm, Em: 668nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant full length protein corresponding to Human PDHA1.

    • Positive control

      • ICC/IF: HeLa cells

    • General notes

       

       

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab197653 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF 1/100.

    Target

    • Function

      The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

    • Tissue specificity

      Ubiquitous.

    • Involvement in disease

      Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
      Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • ODPA_HUMAN antibody
        • PDH antibody
        • PDHA antibody
        • PDHA1 antibody
        • PDHCE1A antibody
        • PDHE1 A type I antibody
        • PDHE1-A type I antibody
        • PHE1A antibody
        • Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody
        • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody
        • Pyruvate Dehydrogenase E1 alpha antibody
        • Pyruvate dehydrogenase E1 component subunit alpha antibody
        • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody
        • Pyruvate Dehydrogenase E1-alpha subunit antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PDHA1 antibody [9H9AF5] (ab197653)
        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PDHA1 antibody [9H9AF5] (ab197653)

        ab197653 staining PDHA1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab197653 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      References (0)

      Publishing research using ab197653? Please let us know so that we can cite the reference in this datasheet.

      ab197653 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab197653.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com