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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)
  • Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR5154] to Peroxiredoxin 2/PRP
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Primary
Product image
Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
Protein
Product image
Recombinant human Peroxiredoxin 2/PRP protein (ab85331)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154]
    See all Peroxiredoxin 2/PRP primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR5154] to Peroxiredoxin 2/PRP
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK293 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5154
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Neurodegenerative disease

Associated products

  • Alternative Versions

    • Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)
    • HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042)
    • Alexa Fluor® 488 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197536)
    • Anti-Peroxiredoxin 2/PRP antibody [EPR5154] - BSA and Azide free (ab227988)
  • Isotype control

    • Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
  • Recombinant Protein

    • Recombinant human Peroxiredoxin 2/PRP protein (ab85331)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
Application Abreviews Notes
ICC/IF
1/100.
Notes
ICC/IF
1/100.

Target

  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P32119 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7001 Human
    • Omim: 600538 Human
    • SwissProt: P32119 Human
    • Unigene: 432121 Human
    • Alternative names

      • Epididymis secretory sperm binding protein Li 2a antibody
      • HEL S 2a antibody
      • MGC4104 antibody
      • Natural killer cell enhancing factor B antibody
      • Natural killer cell-enhancing factor B antibody
      • Natural Killer Enhancing Factor B antibody
      • NKEF B antibody
      • NKEF-B antibody
      • NKEFB antibody
      • Peroxiredoxin 2 antibody
      • Peroxiredoxin-2 antibody
      • PRDX 2 antibody
      • PRDX2 antibody
      • PRDX2_HUMAN antibody
      • PrP antibody
      • PRX2 antibody
      • PRXII antibody
      • PTX1 antibody
      • TDPX1 antibody
      • Thiol Specific Antioxidant 1 antibody
      • Thiol specific antioxidant protein antibody
      • Thiol-specific antioxidant protein antibody
      • Thioredoxin Dependent Peroxide Reductase 1 antibody
      • Thioredoxin peroxidase 1 antibody
      • Thioredoxin-dependent peroxide reductase 1 antibody
      • Torin antibody
      • TPX1 antibody
      • TSA antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)

      ab197041 staining Peroxiredoxin in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab197041 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI. This product also gave a positive signal under the same testing conditions in Hek293 cells fixed with 4% formaldehyde (10 min).
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)
      Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197041)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab197041? Please let us know so that we can cite the reference in this datasheet.

    ab197041 has not yet been referenced specifically in any publications.

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