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Cardiovascular Hypoxia Prolyl Hydroxylase
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RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)

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  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)
  • Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR17869] to PHD3
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

You may also be interested in

Protein
Product image
Recombinant Human PHD3 protein (ab128449)
Conjugation
Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

View more associated products

Overview

  • Product name

    Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869]
    See all PHD3 primary antibodies
  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR17869] to PHD3
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein within Human PHD3 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9H6Z9

  • Positive control

    • ICC/IF: A549 cells CoCl2 treated (0.5mM, 6hr)
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17869
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Prolyl Hydroxylase
    • Epigenetics and Nuclear Signaling
    • Cardiovascular/Immune
    • Hypoxia
    • Prolyl hydroxylase
    • Cancer
    • Invasion/microenvironment
    • Hypoxia
    • Hydroxylases
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-PHD3 antibody [EPR17869] (ab184714)
    • Anti-PHD3 antibody [EPR17869] - BSA and Azide free (ab238941)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human PHD3 protein (ab128449)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF
1/1000.

This product gave a positive signal in A549 cells CoCl2 treated (0.5mM, 6hr) fixed with 100% methanol (5 min)

Notes
ICC/IF
1/1000.

This product gave a positive signal in A549 cells CoCl2 treated (0.5mM, 6hr) fixed with 100% methanol (5 min)

Target

  • Function

    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
  • Tissue specificity

    Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
  • Sequence similarities

    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9H6Z9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 112399 Human
    • Entrez Gene: 112407 Mouse
    • Entrez Gene: 54702 Rat
    • Omim: 606426 Human
    • SwissProt: Q9H6Z9 Human
    • SwissProt: Q91UZ4 Mouse
    • SwissProt: Q62630 Rat
    • Unigene: 135507 Human
    • Unigene: 133037 Mouse
    • Unigene: 10994 Rat
    see all
  • Alternative names

    • Egl 9 family hypoxia inducible factor 3 antibody
    • Egl nine homolog 3 (C. elegans) antibody
    • Egl nine homolog 3 antibody
    • Egl nine like protein 3 isoform antibody
    • EGL9 homolog of C. elegans 3 antibody
    • EGLN3 antibody
    • EGLN3_HUMAN antibody
    • Factor responsive smooth muscle protein antibody
    • HIF Prolyl Hydroxylase 3 antibody
    • HIF-PH3 antibody
    • HIF-prolyl hydroxylase 3 antibody
    • HIFP4H3 antibody
    • HIFPH3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 3 antibody
    • P4H3 antibody
    • PHD3 antibody
    • Prolyl Hydroxylase Domain Containing Protein 3 antibody
    • Prolyl hydroxylase domain-containing protein 3 antibody
    • SM20 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)

    ab225327 staining PHD3 in A549 cells CoCl2 treated (0.5mM, 6hr). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225327 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)
    Alexa Fluor® 647 Anti-PHD3 antibody [EPR17869] (ab225327)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab225327? Please let us know so that we can cite the reference in this datasheet.

ab225327 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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