RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)
  • Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR2580(2)] to PIAS1
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)]
    See all PIAS1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR2580(2)] to PIAS1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).
Notes
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min).

Target

  • Function

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.

  • Tissue specificity

    Expressed in numerous tissues with highest level in testis.

  • Pathway

    Protein modification; protein sumoylation.

  • Sequence similarities

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.

  • Domain

    The LXXLL motif is a transcriptional coregulator signature.
    The SP-RING-type domain is required for promoting EKLF sumoylation.

  • Post-translational
    modifications

    Sumoylated.
    Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.

  • Cellular localization

    Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • AR interacting protein antibody
      • DDXBP1 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
      • DEAD/H box binding protein 1 antibody
      • DEAD/H box-binding protein 1 antibody
      • E3 SUMO-protein ligase PIAS1 antibody
      • GBP antibody
      • Gu binding protein antibody
      • Gu-binding protein antibody
      • GU/RH-II antibody
      • Pias1 antibody
      • PIAS1_HUMAN antibody
      • Protein inhibitor of activated STAT protein 1 antibody
      • Protein inhibitor of activated STAT, 1 antibody
      • RNA helicase II binding protein antibody
      • RNA helicase II-binding protein antibody
      • Zinc finger, MIZ-type containing 3 antibody
      • ZMIZ3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)

      ab203328 staining PIAS1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203328 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)
      Alexa Fluor® 647 Anti-PIAS1 antibody [EPR2580(2)] (ab203328)

    References (0)

    Publishing research using ab203328? Please let us know so that we can cite the reference in this datasheet.

    ab203328 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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