RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)
  • Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR10138(B)] to PKM
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)]
    See all PKM primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR10138(B)] to PKM

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig

  • Immunogen

    Synthetic peptide within Human PKM aa 50-150. The exact sequence is proprietary.
    Database link: P14618

  • Positive control

    • ICC/IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab214257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

  • Tissue specificity

    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

  • Sequence similarities

    Belongs to the pyruvate kinase family.

  • Post-translational
    modifications

    ISGylated.
    Under hypoxia, hydroxylated by EGLN3.
    Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
    FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.

  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
  • Information by UniProt

  • Database links

    • Alternative names

      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • KPYM_HUMAN antibody
      • OIP-3 antibody
      • Opa-interacting protein 3 antibody
      • p58 antibody
      • pkm antibody
      • PKM1 antibody
      • PKM2 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase muscle isozyme antibody
      • Pyruvate kinase PKM antibody
      • THBP1 antibody
      • Thyroid hormone-binding protein 1 antibody
      • Tumor M2-PK antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)

      Ab214257 staining PKM in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab214257 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

       

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

       

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

    • Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)
      Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)] (ab214257)

    References (0)

    Publishing research using ab214257? Please let us know so that we can cite the reference in this datasheet.

    ab214257 has not yet been referenced specifically in any publications.

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