RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)

Submit a review Submit a question
Flow Cytometry - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)
  • Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR17792] to Renilla Luciferase
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792]
    See all Renilla Luciferase primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR17792] to Renilla Luciferase

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: 293T cells transfected with Renilla Luciferase. Flow Cyt: 293T cells transfected with RFP tagged Renilla Luciferase.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17792

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
All applications
Other species
Application Abreviews Notes
Flow Cyt
1/500.
ICC/IF
1/100.

This product gave a positive signal in 293T cells transfected with Renilla Luciferase fixed with 4% formaldehyde (10 min)
Notes
Flow Cyt
1/500.
ICC/IF
1/100.

This product gave a positive signal in 293T cells transfected with Renilla Luciferase fixed with 4% formaldehyde (10 min)

Target

  • Relevance

    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.

  • Database links

    • Alternative names

      • Renilla luciferin 2 monooxygenase antibody
      • Renilla type luciferase antibody

    Images

    • Flow Cytometry - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)
      Flow Cytometry - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)

      Flow cytometric analysis of 293T transfected with RFP tagged Renilla Luciferase stained with ab225339 (right panel). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225339, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (left panel) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Co-staining with RFP (y-axis).

      Acquisition of >5,000 events were collected using a 17 mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)

      ab225339 staining Renilla Luciferase in 293T cells transfected with Renilla Luciferase and normal 293T cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225339 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)
      Alexa Fluor® 647 Anti-Renilla Luciferase antibody [EPR17792] (ab225339)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab225339? Please let us know so that we can cite the reference in this datasheet.

    ab225339 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab225339.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com