Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)](https://www.abcam.com/ps/products/168/ab168536/Images/ab168536-274107-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-647-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [2E3GC12FB2AE2] to SDHA
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Dog, Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2]
See all SDHA primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [2E3GC12FB2AE2] to SDHA -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Dog, Human -
Immunogen
Full length native protein (purified) corresponding to Cow SDHA.
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Positive control
- HeLa and HDFn cells.
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General notes
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
2E3GC12FB2AE2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| Flow Cyt | Use a concentration of 0.2 µg/ml. ab176103 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB | 1/5000. |
Target
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Function
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. -
Involvement in disease
Defects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 281480 Cow
- Entrez Gene: 478634 Dog
- Entrez Gene: 6389 Human
- Entrez Gene: 66945 Mouse
- Entrez Gene: 157074 Rat
- Omim: 600857 Human
- SwissProt: P31039 Cow
- SwissProt: P31040 Human
see all -
Alternative names
- CMD1GG antibody
- DHSA_HUMAN antibody
- Flavoprotein subunit of complex II antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)
ab168536 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab168536 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Western blot - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)](https://www.abcam.com/ps/products/168/ab168536/Images/ab168536-365983-antisdhaantibody2e3gc12fb2ae2hrpwesternblotknockouthek293ogsdhaogmcf7hepg2humanlysates.jpg)
Western blot - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)Clone 2E3GC12FB2AE2 has been knock-out validated in its unconjugated form. Please refer to ab14715 datasheet for experimental details.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)](https://www.abcam.com/ps/products/168/ab168536/Images/ab168536-166931-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)
Immunofluorescent analysis of HeLa cells labeling SDHA (40x).
A) HeLa cells labeled with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Dyelight-594 (1/1000, ab96897).
B) HeLa cells labeled with anti-SDHA Alexa-647 antibody (ab168536 at 1.0 µg/mL.)
C) Merge of A and B to show specificity of signal to mitochondria. -
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)](https://www.abcam.com/ps/products/168/ab168536/Images/ab168536-166930-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)Immunofluorescent analysis of HDFn cells (40x).
A) HDFn stained with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti rabbit Dyelight-594 (1/1000, ab96897).
B) HDFn stained with anti-SDHA Alexa-647 antibody (ab168536 at 1.0 µg/mL).
C) Merge of A and B to show specificity of signal to mitochondria. -
![Flow Cytometry - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)](https://www.abcam.com/ps/products/168/ab168536/Images/ab168536-166929-anti-sdha-antibody-2e3gc12fb2ae2-alexa-fluor-647-flow-cytometry.gif)
Flow Cytometry - Alexa Fluor® 647 Anti-SDHA antibody [2E3GC12FB2AE2] (ab168536)Flow Cytometric analysis of HeLa cells labeling SDHA with ab168536 at 0.2 µg/mL (red) compared with unstained cells (black).
Protocols
Datasheets and documents
References (0)
ab168536 has not yet been referenced specifically in any publications.
