Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
  • Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EPR5984] to TAK1
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984]
    See all TAK1 primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EPR5984] to TAK1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. within Human TAK1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O43318

  • Positive control

    • ICC/IF: differentiated Neuro2a and MCF7 cells, HAP1 cells (WT and MAP3K7-KO).

  • General notes

    Alternative versions available:
    Anti-TAK1 antibody [EPR5984] (ab109526) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab199473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in differentiated Neuro2a and MCF7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
    Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • M3K7_HUMAN antibody
    • MAP3K 7 antibody
    • Map3k7 antibody
    • MEKK7 antibody
    • Mitogen activated protein kinase kinase kinase 7 antibody
    • Mitogen-activated protein kinase kinase kinase 7 antibody
    • TAK1 antibody
    • TGF beta activated kinase 1 antibody
    • TGF-beta-activated kinase 1 antibody
    • TGF1a antibody
    • Transforming growth factor beta activated kinase 1 antibody
    • Transforming growth factor-beta-activated kinase 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)

    ab199473 staining TAK1 in wild-type HAP1 cells (top panel) and MAP3K7 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab199473 at 1/250 dilution and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)

    ab199473 staining TAK1 in differentiated Neuro2a cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199473 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in differentiated Neuro2a cells fixed with 4% formaldehyde (10 min).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)

    ab199473 staining TAK1 in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199473 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 4% formaldehyde (10 min).

  • Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)
    Alexa Fluor® 647 Anti-TAK1 antibody [EPR5984] (ab199473)

References (0)

Publishing research using ab199473? Please let us know so that we can cite the reference in this datasheet.

ab199473 has not yet been referenced specifically in any publications.

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