Key features and details
- Alexa Fluor® 647 Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG2a
Product nameAlexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker
See all Tubulin primary antibodies
DescriptionAlexa Fluor® 647 Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.
- ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
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Our Abpromise guarantee covers the use of ab195884 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsUndergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
ab195884 staining Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195884 at a 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
ab195884 has been referenced in 2 publications.
- Khan MS et al. Glymphatic clearance of simulated silicon dispersion in mouse brain analyzed by laser induced breakdown spectroscopy. Heliyon 6:e03702 (2020). PubMed: 32322711
- Abramo K et al. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol 21:1393-1402 (2019). PubMed: 31685986