RecombinantRabMAb

Recombinant Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)

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Flow Cytometry - Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)
  • Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 647 Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Overview

  • Product name

    Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y]
    See all Vitamin D Binding protein primary antibodies

  • Description

    Alexa Fluor® 647 Rabbit monoclonal [EP2923Y] to Vitamin D Binding protein

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: THP-1 cells. Flow Cyt: Jurkat cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
Application Abreviews Notes
Flow Cyt
1/50.
ICC/IF
1/100.

This product gave a positive signal in THP-1 cells fixed with 100% methanol (5 min).
Notes
Flow Cyt
1/50.
ICC/IF
1/100.

This product gave a positive signal in THP-1 cells fixed with 100% methanol (5 min).

Target

  • Function

    Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)
      Flow Cytometry - Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)
      Overlay histogram showing Jurkat cells stained with ab198339 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198339, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)

      ab198339 staining Vitamin D Binding protein in THP-1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at 1/100 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)
      Alexa Fluor® 647 Anti-Vitamin D Binding protein antibody [EP2923Y] (ab198339)

    References (0)

    Publishing research using ab198339? Please let us know so that we can cite the reference in this datasheet.

    ab198339 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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