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Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)

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Western blot - Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)

    Key features and details

    • Alexa Fluor® 680 Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 680. Ex: 679nm, Em: 702nm
    • Isotype: IgG2b

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    Overview

    • Product name

      Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control
      See all GAPDH primary antibodies
    • Description

      Alexa Fluor® 680 Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 680. Ex: 679nm, Em: 702nm
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Positive control

      • This antibody gave a positive signal in HeLa Nuclear as well as the following whole cell lysates: A431; HEK293; Jurkat.
    • General notes

      According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      mAbcam 9484
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Huntington's disease
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Isotype/Loading Controls
      • Loading Controls
      • GAPDH
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Neurodegenerative disease

    Associated products

    • Alternative Versions

      • Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)
      • Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184578)
      • Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)
      • HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
      • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    • Recombinant Protein

      • Recombinant human GAPDH protein (Active) (ab82633)

    Applications

    Our Abpromise guarantee covers the use of ab184095 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

    Target

    • Function

      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similarities

      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications

      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Target information above from: UniProt accession P04406 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2597 Human
      • Omim: 138400 Human
      • SwissProt: P04406 Human
      • Unigene: 544577 Human
      • Unigene: 592355 Human
      • Unigene: 598320 Human
      • Alternative names

        • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
        • aging associated gene 9 protein antibody
        • Aging-associated gene 9 protein antibody
        • BARS-38 antibody
        • cb609 antibody
        • EC 1.2.1.12 antibody
        • Epididymis secretory sperm binding protein Li 162eP antibody
        • G3P_HUMAN antibody
        • G3PD antibody
        • G3PDH antibody
        • GAPD antibody
        • GAPDH antibody
        • Glyceraldehyde 3 phosphate dehydrogenase antibody
        • glyceraldehyde 3-PDH antibody
        • Glyceraldehyde-3-phosphate dehydrogenase antibody
        • HEL-S-162eP antibody
        • KNC-NDS6 antibody
        • MGC102544 antibody
        • MGC102546 antibody
        • MGC103190 antibody
        • MGC103191 antibody
        • MGC105239 antibody
        • MGC127711 antibody
        • MGC88685 antibody
        • OCAS, p38 component antibody
        • OCT1 coactivator in S phase, 38-KD component antibody
        • peptidyl cysteine S nitrosylase GAPDH antibody
        • Peptidyl-cysteine S-nitrosylase GAPDH antibody
        • wu:fb33a10 antibody
        see all

      Images

      • Western blot - Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)
        Western blot - Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)
        All lanes : Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
        Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
        Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 37 kDa
        Observed band size: 37 kDa



        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184095 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab184095? Please let us know so that we can cite the reference in this datasheet.

      ab184095 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab184095.
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