Alexa Fluor® 700 Anti-CD24 antibody [SN3] (ab269310)
![Flow Cytometry - Alexa Fluor® 700 Anti-CD24 antibody [SN3] (ab269310)](https://www.abcam.com/ps/products/269/ab269310/Images/ab269310-1-AntiCD24AlexaFluor700antibodyHumanflow-cytometry.jpg "Flow Cytometry - Alexa Fluor® 700 Anti-CD24 antibody [SN3] (ab269310)")
Key features and details
- Alexa Fluor® 700 Mouse monoclonal [SN3] to CD24
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 700
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 700 Anti-CD24 antibody [SN3]
See all CD24 primary antibodies -
Description
Alexa Fluor® 700 Mouse monoclonal [SN3] to CD24 -
Host species
Mouse -
Conjugation
Alexa Fluor® 700 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human CD24. Glycoproteins purified from human NALM-1 cell line.
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Positive control
- Flow cyt: Human peripheral blood cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography. -
Clonality
Monoclonal -
Clone number
SN3 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 4µl for 106 cells.
Or 100 μL of whole blood. |
| Notes |
|---|
|
Flow Cyt
Use 4µl for 106 cells. Or 100 μL of whole blood. |
Target
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Function
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. -
Tissue specificity
B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. -
Involvement in disease
Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. -
Sequence similarities
Belongs to the CD24 family. -
Post-translational
modificationsExtensively O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100133941 Human
- Omim: 600074 Human
- SwissProt: P25063 Human
- Unigene: 644105 Human
- Unigene: 721443 Human
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Alternative names
- CD 24 antibody
- CD24 antibody
- CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 700 Anti-CD24 antibody [SN3] (ab269310)Flow cytometry analysis (surface staining) of human peripheral blood cells labeling CD24 with ab269310.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab269310 has not yet been referenced specifically in any publications.