Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
![Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)](https://www.abcam.com/ps/products/270/ab270669/Images/ab270669-381758-anticd34alexafluor700-antibody-4h11apg-flowdensity-human-peripheral-whole-blood.JPG "Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)")
Key features and details
- Alexa Fluor® 700 Mouse monoclonal [4H11[APG]] to CD34
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 700
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]]
See all CD34 primary antibodies -
Description
Alexa Fluor® 700 Mouse monoclonal [4H11[APG]] to CD34 -
Host species
Mouse -
Conjugation
Alexa Fluor® 700 -
Specificity
This antibody reacts with extracellular class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
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Positive control
- Flow Cyt: Human peripheral whole blood.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
4H11[APG] -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 4µl for 106 cells.
The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension. |
| Notes |
|---|
|
Flow Cyt
Use 4µl for 106 cells. The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)
Flow cytometry analysis of human peripheral whole blood labeling CD34 using ab270669. Surface staining.
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![Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)](https://www.abcam.com/ps/products/270/ab270669/Images/ab270669-381759-anticd34alexafluor700-antibody-4h11apg-flowhistogram-human-peripheral-whole-blood.JPG)
Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)Flow cytometry analysis of human peripheral whole blood labeling CD34 positive stem cells using ab270669 (Red).
Control: Lymphocytes (Black).
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![Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)](https://www.abcam.com/ps/products/270/ab270669/Images/ab270669-381760-anticd34alexafluor700-antibody-4h11apg-flowdensity-human-peripheral-whole-blood-2.JPG)
Flow Cytometry - Alexa Fluor® 700 Anti-CD34 antibody [4H11[APG]] (ab270669)Flow cytometry analysis of human peripheral whole blood labeling CD34 positive stem cells using ab270669 (Red). Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab270669 has not yet been referenced specifically in any publications.
