Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
![Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)](https://www.abcam.com/ps/products/270/ab270671/Images/ab270671-381751-anticd4alexafluor700-antibody-mem241-flowhistogram-human-peripheral-whole-blood.JPG "Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)")
Key features and details
- Alexa Fluor® 700 Mouse monoclonal [MEM-241] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 700
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 700 Anti-CD4 antibody [MEM-241]
See all CD4 primary antibodies -
Description
Alexa Fluor® 700 Mouse monoclonal [MEM-241] to CD4 -
Host species
Mouse -
Conjugation
Alexa Fluor® 700 -
Specificity
This antibody recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes and also on monocytes, tissue macrophages and granulocytes.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to CD4. 2 N-terminal domains of human CD4 fused to human IgG1 Fc.
Database link: P01730 -
Positive control
- Flow Cyt: Human peripheral whole blood.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-241 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 4µl for 106 cells.
The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension. |
| Notes |
|---|
|
Flow Cyt
Use 4µl for 106 cells. The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
Flow cytometry analysis of human peripheral whole blood labeling CD4 positive lymphocytes using ab270671 (Red). Surface staining.
Control: Neutrophil granulocytes (Black).
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![Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)](https://www.abcam.com/ps/products/270/ab270671/Images/ab270671-381750-anticd4alexafluor700-antibody-mem241-flowdensity-human-peripheral-whole-blood.JPG)
Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)Flow cytometry analysis of human peripheral whole blood labeling CD4 using ab270671. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab270671 has not yet been referenced specifically in any publications.
