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    alexa-fluor-700-cd4-antibody-mem-241-ab270671.pdf

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Immunology Adaptive Immunity T Cells CD
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Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)

  • Datasheet
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Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
  • Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)

Key features and details

  • Alexa Fluor® 700 Mouse monoclonal [MEM-241] to CD4
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 700
  • Isotype: IgG1

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Overview

  • Product name

    Alexa Fluor® 700 Anti-CD4 antibody [MEM-241]
    See all CD4 primary antibodies
  • Description

    Alexa Fluor® 700 Mouse monoclonal [MEM-241] to CD4
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 700
  • Specificity

    This antibody recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes and also on monocytes, tissue macrophages and granulocytes.

  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to CD4. 2 N-terminal domains of human CD4 fused to human IgG1 Fc.
    Database link: P01730

  • Positive control

    • Flow Cyt: Human peripheral whole blood.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Clonality

    Monoclonal
  • Clone number

    MEM-241
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Alternative Versions

    • APC Anti-CD4 antibody [MEM-241] (ab18280)
    • FITC Anti-CD4 antibody [MEM-241] (ab18281)
    • PE Anti-CD4 antibody [MEM-241] (ab18282)
    • Biotin Anti-CD4 antibody [MEM-241] (ab28069)
    • PerCP Anti-CD4 antibody [MEM-241] (ab65951)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 4µl for 106 cells.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.

Notes
Flow Cyt
Use 4µl for 106 cells.

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 920 Human
    • Omim: 186940 Human
    • SwissProt: P01730 Human
    • Unigene: 631659 Human
    • Alternative names

      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Images

    • Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
      Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)

      Flow cytometry analysis of human peripheral whole blood labeling CD4 positive lymphocytes using ab270671 (Red). Surface staining.

      Control: Neutrophil granulocytes (Black).

    • Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
      Flow Cytometry - Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)

      Flow cytometry analysis of human peripheral whole blood labeling CD4 using ab270671. Surface staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab270671? Please let us know so that we can cite the reference in this datasheet.

    ab270671 has not yet been referenced specifically in any publications.

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