Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)
![Flow Cytometry - Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)](https://www.abcam.com/ps/products/270/ab270668/Images/ab270668-381766-anticd80alexafluor700-antibody-mem233-flowhiogram-human-CD80transfected-p815-cells.JPG "Flow Cytometry - Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)")
Key features and details
- Alexa Fluor® 700 Mouse monoclonal [MEM-233] to CD80
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 700
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 700 Anti-CD80 antibody [MEM-233]
See all CD80 primary antibodies -
Description
Alexa Fluor® 700 Mouse monoclonal [MEM-233] to CD80 -
Host species
Mouse -
Conjugation
Alexa Fluor® 700 -
Specificity
This antibody recognizes an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD80 (extracellular). Fused to human IgG1 (Fc).
Database link: P33681 -
Positive control
- Flow Cyt: CD80-transfected P815 cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-233 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- FITC Anti-CD80 antibody [MEM-233] (ab18279)
- PE/Cy5® Anti-CD80 antibody [MEM-233] (ab233572)
- PE/Cy7® Anti-CD80 antibody [MEM-233] (ab234229)
- APC Anti-CD80 antibody [MEM-233] (ab27554)
- PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)
- Anti-CD80 antibody [MEM-233] (ab8239)
- PerCP Anti-CD80 antibody [MEM-233] (ab82410)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 4µl for 106 cells.
The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.
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| Notes |
|---|
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Flow Cyt
Use 4µl for 106 cells. The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.
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Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)
Flow cytometry analysis of human CD80-transfected P815 cells labeling CD80 using ab270668. Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab270668 has not yet been referenced specifically in any publications.
