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Immunology Adaptive Immunity B Cells CD
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Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)

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Flow Cytometry - Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)

    Key features and details

    • Alexa Fluor® 700 Mouse monoclonal [MEM-233] to CD80
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Alexa Fluor® 700
    • Isotype: IgG1

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    Overview

    • Product name

      Alexa Fluor® 700 Anti-CD80 antibody [MEM-233]
      See all CD80 primary antibodies
    • Description

      Alexa Fluor® 700 Mouse monoclonal [MEM-233] to CD80
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 700
    • Specificity

      This antibody recognizes an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

    • Tested Applications & Species

      Application Species
      Flow Cyt (Intra)
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Recombinant fragment corresponding to Human CD80 (extracellular). Fused to human IgG1 (Fc).
      Database link: P33681

    • Positive control

      • Flow Cyt: CD80-transfected P815 cells.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      MEM-233
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • FITC Anti-CD80 antibody [MEM-233] (ab18279)
      • PE/Cy5® Anti-CD80 antibody [MEM-233] (ab233572)
      • PE/Cy7® Anti-CD80 antibody [MEM-233] (ab234229)
      • APC Anti-CD80 antibody [MEM-233] (ab27554)
      • PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)
      • Anti-CD80 antibody [MEM-233] (ab8239)
      • PerCP Anti-CD80 antibody [MEM-233] (ab82410)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab270668 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt (Intra)
    Recombinant fragment
    Application Abreviews Notes
    Flow Cyt
    Use 4µl for 106 cells.

    The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.

     

    Notes
    Flow Cyt
    Use 4µl for 106 cells.

    The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in suspension.

     

    Target

    • Function

      Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
    • Tissue specificity

      Expressed on activated B-cells, macrophages and dendritic cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P33681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 941 Human
      • Omim: 112203 Human
      • SwissProt: P33681 Human
      • Unigene: 838 Human
      • Alternative names

        • Activation B7-1 antigen antibody
        • B lymphocyte activation antigen B7 antibody
        • B7 antibody
        • B7-1 antibody
        • B7-1 antigen antibody
        • B7.1 antibody
        • BB1 antibody
        • CD28 antigen ligand 1 antibody
        • CD28LG antibody
        • CD28LG1 antibody
        • CD80 antibody
        • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
        • CD80 antigen antibody
        • CD80 molecule antibody
        • CD80_HUMAN antibody
        • Costimulatory factor CD80 antibody
        • costimulatory molecule variant IgV-CD80 antibody
        • CTLA-4 counter-receptor B7.1 antibody
        • LAB7 antibody
        • T-lymphocyte activation antigen CD80 antibody
        see all

      Images

      • Flow Cytometry - Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)
        Flow Cytometry - Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)

        Flow cytometry analysis of human CD80-transfected P815 cells labeling CD80 using ab270668. Surface staining.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab270668? Please let us know so that we can cite the reference in this datasheet.

      ab270668 has not yet been referenced specifically in any publications.

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