For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    alexa-fluor-790-alpha-tubulin-antibody-dm1a-loading-control-ab184577.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Isotype/Loading Controls Loading Controls Tubulin
Share by email

Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)

  • Datasheet
  • SDS
Reviews (2) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)

    Key features and details

    • Alexa Fluor® 790 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Conjugation: Alexa Fluor® 790. Ex: 782nm, Em: 805nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 680 Carrier Free FITC HRP Unconjugated

    You may also be interested in

    Protein
    Product image
    Recombinant Human beta I Tubulin protein (ab164310)
    Primary
    Product image
    HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)

    View more associated products

    Overview

    • Product name

      Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control
      See all alpha Tubulin primary antibodies
    • Description

      Alexa Fluor® 790 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
    • Host species

      Mouse
    • Conjugation

      Alexa Fluor® 790. Ex: 782nm, Em: 805nm
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
    • Epitope

      aa 426-450
    • Positive control

      • This antibody gave a positive signal in the following whole cell lysates: HeLa; MCF7; U2OS; NIH3T3.
    • General notes

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      DM1A
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Isotype/Loading Controls
      • Loading Controls
      • Tubulin
      • Tags & Cell Markers
      • Subcellular Markers
      • Cytoskeleton
      • Microtubules
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • Tubulin
      • Isotype/Loading Controls
      • Loading Controls
      • Alpha Tubulin
      • Neuroscience
      • Processes

    Associated products

    • Alternative Versions

      • Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
      • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
      • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
      • Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
      • HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
      • FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
      • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab184577 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (2)
    Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

    Target

    • Function

      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • Sequence similarities

      Belongs to the tubulin family.
    • Post-translational
      modifications

      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P68366 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7277 Human
      • Entrez Gene: 22145 Mouse
      • Omim: 191110 Human
      • SwissProt: P68366 Human
      • SwissProt: P68368 Mouse
      • Unigene: 75318 Human
      • Unigene: 1155 Mouse
      • Alternative names

        • Alpha-tubulin 1 antibody
        • ALS22 antibody
        • B ALPHA 1 antibody
        • bA408E5.3 antibody
        • H2 ALPHA antibody
        • Hum a tub1 antibody
        • Hum a tub2 antibody
        • LIS3 antibody
        • MGC171407 antibody
        • MGC55332 antibody
        • TBA4A_HUMAN antibody
        • Testis-specific alpha-tubulin antibody
        • TUBA1 antibody
        • TUBA1A antibody
        • tuba1l antibody
        • Tuba4a antibody
        • Tubulin alpha 1 chain antibody
        • Tubulin alpha antibody
        • Tubulin alpha-1 chain antibody
        • tubulin alpha-1B chain antibody
        • Tubulin alpha-4A chain antibody
        • Tubulin H2-alpha antibody
        • Tubulin, alpha 1 (testis specific) antibody
        • tubulin, alpha 1, like antibody
        • Tubulin, alpha 4a antibody
        • Tubulin, alpha, testis-specific antibody
        • Tubulin, alpha-1 antibody
        see all

      Images

      • Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
        Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
        All lanes : Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
        Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
        Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 52 kDa
        Observed band size: 52 kDa



        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184577 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab184577? Please let us know so that we can cite the reference in this datasheet.

      ab184577 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (mouse ES cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      mouse ES cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Mar 18 2021

      Western blot abreview for Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (eHAP cells)
      Gel Running Conditions
      Reduced Denaturing (4-10% gradient gel)
      Loading amount
      5 µg
      Specification
      eHAP cells
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Mar 04 2021

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2023 Abcam plc. All rights reserved.