Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)

    Overview

    • Product name

      Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
      See all IgG isotype controls

    • Conjugation

      Alexa Fluor® 594. Ex: 590nm, Em: 617nm

    • Tested applications

      Suitable for: ICC/IFmore details

    • General notes

      Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The Control performed appropriately in all materials and platforms that were tested. 

      Product was previously marketed under the MitoSciences sub-brand.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.

    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...

    • Purity

      Ammonium Sulphate Precipitation

    • Clonality

      Monoclonal

    • Clone number

      15-6E10A7

    • Isotype

      IgG1

    • Light chain type

      kappa

    • Research areas

    • Cellular localization

      Secreted

    • Alternative names

      • Ig gamma 1 chain C region
      • Ig gamma 2 chain C region
      • Ig gamma 3 chain C region
      • Ig gamma 4 chain C region
      • IgG
      • IGHG1
      • IGHG2
      • IGHG3
      • IGHG4
      • Immunoglobin heavy constant gamma 1
      • Immunoglobulin G
      see all

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab178000 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab178000)

      Immunocytochemistry data for ab178000. All panels show HeLa cells stained with DAPI as well as; a no primary control (A), ab178000 at 0.1 μg/mL (B), an Alexa 594 anti-SDHA conjugated antibody (ab170172) at 0.1 μg/mL (C).

      Cells were PFA fixed and stored in 0.02%azide/PBS in 4°C, then blocked with 10%GS for 2 hours, primary was diluted in 10%GS and incubated for 2 hours, cells were washed 3x with 1%GS and DAPI was added for 10 minutes in 1%GS.

    References (0)

    Publishing research using ab178000? Please let us know so that we can cite the reference in this datasheet.

    ab178000 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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